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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
The occurrence of cryptosporidia in children
 MACHOVCOVÁ, Petra
The aim of this thesis was to determine the incidence of unicellular parasites of the genus Cryptosporidium, which is the causative agent of cryptosporidiosis in children. 67 stool samples of children at the age of 1-15 were analyzed in this study. Out of it, 4 children were admitted to the infectious ward of the České Budějovice Hospital, Corp. Other samples were taken due to the suspicion of pathogene presence which causes these disorders, mainly abdominal pain, diarrhoea and fever. The samples were analyzed microscopically with the sedimentation method M.I.F.C., which serves for the intestinal parasite diagnosis, along with the staining method according to Miláček ?Vítovec, which is used to prove oocyst crystosporidia in the stool. They were also analyzed molecularly with the help of PCR. The cryptosporidia oocysts were not detected in any sample.
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Occurrence of microsporidia found in various groups of patients (children)
 SMETANOVÁ, Renáta
The objective of this study was to identify the occurrence of microsporidia in children. The children may be endangered by microsporidia due to immune system deficiency. The stool specimens were collected from children hospitalized with various diagnoses in the hospital České Budějovice. These samples were collected from children of both sexes aged 0 ? 15 years. Diagnostic methods included fluorescence and light microscopy and a molecular technique (PCR). Concentration sedimentation method was used to diagnose eggs, larvae and oocysts of any other parasites in the stool. Calcofluor, a special fluorescent stain, was used to diagnose microsporidia spores. Molecular techniques (PCR) were used for diagnostics of of microsporidia species found in the stool samples. None of the stool samples of the children was positive.
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