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Occurrence of microsporidia found in various groups of patients (children)
SMETANOVÁ, Renáta
The objective of this study was to identify the occurrence of microsporidia in children. The children may be endangered by microsporidia due to immune system deficiency. The stool specimens were collected from children hospitalized with various diagnoses in the hospital České Budějovice. These samples were collected from children of both sexes aged 0 ? 15 years. Diagnostic methods included fluorescence and light microscopy and a molecular technique (PCR). Concentration sedimentation method was used to diagnose eggs, larvae and oocysts of any other parasites in the stool. Calcofluor, a special fluorescent stain, was used to diagnose microsporidia spores. Molecular techniques (PCR) were used for diagnostics of of microsporidia species found in the stool samples. None of the stool samples of the children was positive.

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