Home > Search Results: keywords:"Importazole"
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 1 records found  Search took 0.02 seconds. 
Nuclear import and export inhibitors analysis in plants
 Haltuf, Josef ; Schwarzerová, Kateřina (advisor) ; Angelini, Jindřiška (referee)
Eukaryotic cells have a nucleus where DNA is stored. Transport between the nucleus and cytoplasm is extremely important for ensuring gene expression as well as for controlling and regulating nuclear processes and structures. During interphase, thousands of macromolecules are transported into and out of the nucleus through nuclear pores, and nucleocytoplasmic transport is essential for the viability of eukaryotic organisms. Transport between the nucleus and cytoplasm is tightly regulated, and specific inhibitors of nuclear transport are used to study this regulation. While nuclear transport inhibitors have been extensively studied in animal cell research, the activity of most of them has not been tested in plant cells. In this work the nuclear export inhibitors Leptomycin B (LMB) and the nuclear import inhibitors Importazole (IMZ) and Ivermectin (IVR) are tested in Arabidopsis thaliana plant roots and in isolated tobacco BY- 2 cells. This work also includes testing suitable markers for transport between the nucleus and cytoplasm. GFP-NES and NLS-GFP were found to be the best markers because they are stable and can diffuse spontaneously through nuclear pores. With these markers, I determined effective concentrations for LMB (100 nM in roots, 50 nM in isolated cells) and IMZ (75 μM in roots). IVR proved to be...
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English