National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Evolution of anaerobic Heterolobosea
Pánek, Tomáš ; Čepička, Ivan (advisor) ; Oborník, Miroslav (referee)
Heterolobosea is a group of mainly free-living amoebae, flagellates and amoeboflagellates closely related to Euglenozoa and Jakobida (supergroup Excavata). At least two heterolobosean lineages have reduced their mitochondria and live in low concentrations of oxygen (Psalteriomonadidae and extremely halophilic Pleurostomum). Moreover, some other presumably anaerobic species have been described in aerobic heteroloboseid genera, but their sequence data are still missing. The aim of this study is to recognize the diversity and phylogenetic position of both already described and novel non-hypersaline anaerobic heteroloboseans. We have isolated and cultivated 34 strains of free-living heterolobosean flagellates, amoebae and amoeboflagellates from freshwater, brackish and marine microoxic/anoxic habitats. 33 of these strains constitute a single clade (Psalteriomonadidae). We recognize 2 new genera and 3 new species. Phylogenetic analysis of SSU rDNA showed the genus Percolomonas is polyphyletic. The similar morphology of species formally placed within the genus Percolomonas is probably plesiomorphic. Finally, the marine strain PC4AM is morphologically unique and its morphology can not be compared with other heteroloboseans and eukaryotes. It is probably the most derived heterolobosean. Its cell structure...
Diversity, phylogeny and phylogeography of free-living amoebae
TYML, Tomáš
This thesis consists of seven published papers on free-living amoebae (FLA), members of Amoebozoa, Excavata: Heterolobosea, and Cercozoa, and covers three main topics: (i) FLA as potential fish pathogens, (ii) diversity and phylogeography of FLA, and (iii) FLA as hosts of prokaryotic organisms. Diverse methodological approaches were used including culture-dependent techniques for isolation and identification of free-living amoebae, molecular phylogenetics, fluorescent in situ hybridization, and transmission electron microscopy.

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