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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Vliv oxidačního stresu na aktivitu telomerických elementů u \kur{Drosophila melanogaster}
 HEJDOVÁ, Veronika
HeTom transgene expression was studied in order to determine the effect of chronic oxidative stress, using paraquat, on transcriptional activity of the promoter of Het-A telomeric element. After exposure to low paraquat concentrations, I found increased transcript levels of telomeric HeT-A as well as HeTom, the non-telomeric insertion of HeT-A promoter in combination with the gene for fluorescent protein Tomato.
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Analýza promotorových sekvencí telomerického elementu \kur{HeT-A} u \kur{Drosophila melanogaster}
 ŠVELLEROVÁ, Hana
Drosophila melanogaster extends its telomeres by transposition of special telomeric retroelements (HeT-A, TART and TAHRE) targeted specifically to chromosome ends. Retroelement HeT-A is the most studied of telomeric elements and recent studies revealed significant sequence variability of the element HeT-A, not only along its length but also in areas with regulatory activity. This thesis is focused on the activity of different HeT-A promotors during the whole Drosophila development and comparision of transgenic lines with HeT-A promotor and reporter Tomato transgen and it was confirmed the sequence variability of the promotor HeT-A element and its tissue and organ specificity.
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Transcriptional analysis of the \kur{HeT-A} retrotransposon in \kur{Drosophila melanogaster}
 SÁBOVÁ, Michala
Instead of using telomerase, Drosophila melanogaster extends its telomeres by transposition of special telomeric retroelements (HeT-A, TART and TAHRE) targeted specifically to chromosome ends. One key step of the transposition mechanism is a transcription of the elements. Using the expression of a reporter Tomato transgene under HeT-A promoter control we obtained a spatial and temporal visualization of HeT-A promoter activity during the whole Drosophila development. This analysis confirmed that the activity of the HeT-A promoter is up-regulated by cell proliferation, however HeT-A promoter activity is not limited only to proliferating diploid cells. One important outcome of this study is the observation of variation in HeT-A promoter activity in both location and intensity.
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