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Ovlivnění plazmatické hladiny cholesterolu modelového organizmu polynenasycenými mastnými kyselinami n-3
Čeganová, Markéta
The aim of this thesis is to assess the influence of the polyunsaturated fatty acids, especially DHA and EPA in the diet on the animal organism in accordance to the levels of total HDL and LDL cholesterol and triglycerides in plasma. As experimental animals were used rats and they were divided into 3 groups of 10 pieces: control group with the addition of 6 % Carthamus tinctorius (n-6 flares), the group with the addition of 6 % fish oil (n-3: EPA, DHA fish) and the group with 6 % addition of oil from Schizochytrium (n-3: DHA, DHA). After 40 days the animals were putted to death according to relevant regulations. Blood lipids were obtained from blood samples by spectrophotometry. Value of polyunsaturated fatty acids of n-3 and n-6 was set by gas chromatography in the liver, muscle and adipose tissue. In accordance to the increase of content n-3 PUFA in group with fish oil and with oil from Schizochytrium, total and LDL cholesterol was reduced in comparison of samples in control group. In comparison to the control group were TAG reduced only in the group with addition of oil.
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Knowledge of plasma lipids of university students, smokers
VARAUSOVÁ, Anežka Gabriela
Introduction: Cardiovascular diseases are among the most common causes of death. The risk factors include, among others, hypercholesterolemia. It has been long believed that a high level of cholesterol intake via meals (dietary cholesterol) has negative effects on the cardiovascular system and therefore it has been deliberately removed from diet. Several studies, however, confirm the assumption that cholesterol in diet (dietary cholesterol) has little effect on cholesterol levels in blood. The human body receives cholesterol through nourishment in form of animal fats, but it can produce it in the liver, too. So if cholesterol is removed from nourishment due to a diet, the liver can cover the need of cholesterol through increased production. Cholesterol level in the human body is influenced by many factors most of which we can control. These factors include food containing too much saturated fat or cholesterol, overweight, physical inactivity, congenital health conditions, age and gender. There are no symptoms of hypercholesterolemia at the first stage and that is why the current population does not pay such attention, which it should deserve, to hypercholesterolemia. Because there are a number of educational materials on this subject, I decided to explore among the university students (especially smokers) if they are interested in this subject and what their knowledge is. Objective: The main goal of my thesis was to map out the university students´ (smokers) knowledge of plasma lipids. The bachelor thesis was prepared by means of a quantitative research using questionnaires. The research sample consisted of 300 full-time students. I collected 270 filled-in questionnaires out of the 300 distributed. The questionnaire return rate was therefore 90%. 99 out of those 270 were unusable due to the fact that the respondents did not belong to the target group. The total number of questionnaires to be evaluated was 171 questionnaires. Results: I defined a research hypothesis. Hypothesis # 1 Knowledge of plasma lipids of university students, smokers is inadequate, has been confirmed. 36 (21%) respondents out of the total 171 respondents knew their level of cholesterol and 135 (79%) of the respondents did not know their level. Conclusion: This thesis can be used in practice as a publication, to increase the general awareness of students and professors of the issue of cholesterol. An educational material is attached to this thesis that can be used in general practitioners? surgeries.
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