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Studium změn antimikrobiální aktivity houby Ganoderma adspersum pomocí kultivační strategie OSMAC
Komárková, Lucie
Ganoderma adspersum is a white rot fungus that degrades lignocellulose in nature. However, it has also been proven to have many biological activities, including antioxidant, antitumor and anti-inflammatory. This study is focused on monitoring the antimicrobial activity of G. adspersum against the bacteria Escherichia coli, Staphylococcus aureus, MRSA and Klebsiella pneumoniae. First, the fungus was cultivated in three media with different additives. Using the minimum inhibitory concentration test, the effect of individual cultivation on viability was monitored. The lowest values were measured in YMB medium with tryptophan, which reduced the viability of E. coli up to 23 %. Based on this result, E. coli was chosen for growth curve, where media with additives were compared with pure media. It was found that pure media showed greater inhibitory activity than media with additives. The highest activity was observed in pure PDB medium, in which nearly 40% inhibition occurred at a concentration of 0,25 mg/ml. The results showed that Ganoderma adspersum could be a potential antimicrobial agent.
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