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Cell Viability Measurement
Prokopyeva, Elena ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
This master's thesis deals with the ways of cell viability determination and its measuring. The first part of this thesis has a brief introduction into the theory of cell viability, also the methods of cell viability measurement and different types of fluorescent probes are described. The thesis further includes a brief description of the image processing methods, as well as the fluorescent microscope and applied filters are described. In the practical part the system implemented at a graphical programming environment LabVIEW used for the cell viability evaluation is described, functions of applied units are explained. The program is checked on model data.
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System for measurement of concentration of intracellular calcium in LabVIEW
Kormanovskaya, Elena ; Kolářová, Jana (referee) ; Čmiel, Vratislav (advisor)
The master’s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis included theoretical and practical parts. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. Literary solution is presented. Block diagram of system for measurement of concentration of intracellular calcium at a graphical programming environment LabVIEW is presented in practical part of master’s thesis.
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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Intracellular Calcium Measurement Methods
Číhal, Martin ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
This work aims to focus on the issue of intracellular calcium. Not only the theory dealing with the history of fluorescent indicators but also the types of fluorescent indicators and methods of measurements are summarized. The individual steps of measurements on the fluorescent microscope are mentioned as well. There is also created a program for evaluting Ca2+ concentrations in cells made in matlab.
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Intracellular Calcium Measurement Methods
Číhal, Martin ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
This work aims to focus on the issue of intracellular calcium. Not only the theory dealing with the history of fluorescent indicators but also the types of fluorescent indicators and methods of measurements are summarized. The individual steps of measurements on the fluorescent microscope are mentioned as well. There is also created a program for evaluting Ca2+ concentrations in cells made in matlab.
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|
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
|
|
|
System for measurement of concentration of intracellular calcium in LabVIEW
Kormanovskaya, Elena ; Kolářová, Jana (referee) ; Čmiel, Vratislav (advisor)
The master’s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis included theoretical and practical parts. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. Literary solution is presented. Block diagram of system for measurement of concentration of intracellular calcium at a graphical programming environment LabVIEW is presented in practical part of master’s thesis.
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|
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Cell Viability Measurement
Prokopyeva, Elena ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
This master's thesis deals with the ways of cell viability determination and its measuring. The first part of this thesis has a brief introduction into the theory of cell viability, also the methods of cell viability measurement and different types of fluorescent probes are described. The thesis further includes a brief description of the image processing methods, as well as the fluorescent microscope and applied filters are described. In the practical part the system implemented at a graphical programming environment LabVIEW used for the cell viability evaluation is described, functions of applied units are explained. The program is checked on model data.
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