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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Testování genetické čistoty hybridního osiva zelí hlávkového pomocí molekulárních metod
 O'Shea, Denisa
This diploma thesis deals with testing of hybrid seeds genetic purity in three varieties of head cabbage (Brassica oleracea var. capitata L.). The suitability of SSR primers chosen according to an earlier screening carried out as a part of a research project in the workplace of Mendeleum and hybrid seeds genetic purity in head cabbage by SSR molecular method was tested. Among the total of 8 SSR primers screened, only 5 could produce both parent specific markers and therefore they were able to differentiate hybrid offspring and its corresponding parental lines, by which crossbreeding they originated. For each variety, 70 samples of hybrid offspring and 2 samples of parental lines were tested always in two sets. By using these primers, the genetic purity of the variety D - F1 was determinated as 100% and of the varieties H - F1 and T - F1 as 98.57%. The results of this thesis have the potential to be used in practice by a commercial company that has provided the seed samples for testing.
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Ověřování SSR markerů vhodných pro rozlišování odrůd vybraných zelenin z čeledi Brassicaceae
 Sochorová, Jana
In available literature there is no recommended set of microsatellite markers in the improvement of cabbage cultivars (Brassica oleracea conv. capitata) to distinguish F1 hybrids and self-pollinated plants with a parental genotype. That is why microsatellite markers used to other Brassicas had been searched in this work. Tested microsatellite markers were studied for their ability of distinguishing F1 hybrids in seven cabbage cultivars. Two of 23 microsatellite markers were able to distinguish F1 hybrids in six cabbage cultivars. No microsatellite marker was able to distinguish F1 hybrid and parental genotypes of one cultivar, 'A'.
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