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The Potential Use of the Eustigmatophyceae In the Production of Biofuels
Pilátová, Jana ; Němcová, Yvonne (advisor) ; Pichrtová, Martina (referee)
The eustigmatophytes - yellow-green microalgae - are well known for their high lipid content which is constituted by high amounts of essential polyunsaturated fatty acids (PUFA), such as eicosapentaenoic acid (EPA). This, combined with their high growth rate and an exceptionally high adaptability, makes them very promising feedstock for the biofuels production. In this bachelor thesis the role of eustigmatophytes in the production of biofuels is depicted with consideration of the issue of various cultivation conditions effects on the lipid content and composition. The first review of fatty acid distribution in eustigmatophytes ever reported is provided herein. Thus this work might be tentatively contributing to the strain selection issue - one of the most discussed topics in the biofuels field. The fatty acid distribution is qualitatively and quantitatively changed under various chemical and physical stress conditions. Thus the impact of nitrogen and phosphorous availability alongside with light intensity or temperature on lipid changes are presented. In addition, the process of fatty acid and polyunsaturated fatty acid synthesis is briefly outlined. Recent genetic engineering trends and successes are also mentioned, such as the implementation of high-efficiency homologous recombination to...
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The Potential Use of the Eustigmatophyceae In the Production of Biofuels
Pilátová, Jana ; Němcová, Yvonne (advisor) ; Pichrtová, Martina (referee)
The eustigmatophytes - yellow-green microalgae - are well known for their high lipid content which is constituted by high amounts of essential polyunsaturated fatty acids (PUFA), such as eicosapentaenoic acid (EPA). This, combined with their high growth rate and an exceptionally high adaptability, makes them very promising feedstock for the biofuels production. In this bachelor thesis the role of eustigmatophytes in the production of biofuels is depicted with consideration of the issue of various cultivation conditions effects on the lipid content and composition. The first review of fatty acid distribution in eustigmatophytes ever reported is provided herein. Thus this work might be tentatively contributing to the strain selection issue - one of the most discussed topics in the biofuels field. The fatty acid distribution is qualitatively and quantitatively changed under various chemical and physical stress conditions. Thus the impact of nitrogen and phosphorous availability alongside with light intensity or temperature on lipid changes are presented. In addition, the process of fatty acid and polyunsaturated fatty acid synthesis is briefly outlined. Recent genetic engineering trends and successes are also mentioned, such as the implementation of high-efficiency homologous recombination to...
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Ultrastructure of eustigmatophycean algae
Fišerová, Melánie ; Němcová, Yvonne (advisor) ; Hyliš, Miroslav (referee)
In 1971, the algal class Eustigmatophyceae, Stramenopiles, was detached by Hibberd and Leedale from the class Xanthophyceae, Stramenopiles, on the basis of prominent ultrastructural differences in vegetative cells and more importantly in zoospores, such as the presence of big extraplastidial stigma. The class was divided into four families, six genera and twelve species. It seems so far, that there is a deep dichotomy within the class. Eliáš et al. (unpublished) recom- mend to establish two orders based on molecular sequencing of 18S rDNA and rbcL: Eustigmatales (sen- su Eustigmatophyceae described by Hibberd) and Goniochloridales (order containing Goniochloris, Pseu- dostaurastrum and undescribed relatives). The order Eustigmatales is divided into three separate lineages, most likely families: Eustigmataceae (A1), Monodopsidaceae (A2) and a new family Pseudellipsoidiona- ceae (A3). In this study the ultrastructure of 10 eustigmatophycean strains from three separate lineages was investigated. The typical characteristics of this class as chloroplast without a girdle lamella, a reddish glo- bule and lamellate vesicles were found in all strains studied. The appearance of the lamellate vesicles was found to change during the life cycle. My research indicates that other characteristics, formerly...
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