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Optical Measurement of Electromechanical Characteristics of Heart Cells
Čmiel, Vratislav ; Križanová,, Olga (referee) ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
Dissertation is focused on the application of optical measurement methods using techniques of optical microscopy and fluorescence microscopy in measurements of electromechanical characteristics of isolated cardiac cells and clusters of differentiated cardiomyocytes. The first proposed method uses a practical combination of fluorescence microscopy equipped with fluorescent fast and high-resolution camera and atomic force microscopy for simultaneous measurement of calcium transients and contraction of cardiomyocyte clusters. The signals obtained undergoes filtration, processing and analysis. Result function parameters obtained by analyzing signals after application of caffeine are evaluated by comparison with functional parameters obtained during the control measurement. The second proposed method is applied to the cardiomyocyte clusters for the purpose of cardiomyocyte contraction signals measurement. The signals obtained by optical methods are analyzed and compared with the reference signal obtained using atomic force microscopy. Optical measurement method of cell contractins based on detection of cell ends using adjusting of microscopy images by re-sharpening and fluorescence method for cardiomyocyte contractions measurements were designed to increase realiability in simultaneous measurement of cell contractions simultaneously with calcium transients in isolated cardiomyocytes experiments.
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Specifics of nursing care for a patient with the disease Epidermolysis bullosa congenita.
ŠÍTALOVÁ, Terezie
This Bachelor's thesis is aimed at the specifics of the nursing caring of patients suffering from congenital epidermolysis bullosa (EB). The thesis is divided into two parts, theoretic and scientific. The theoretic part describes the diagnosis and therapy of the disease. The main focus of this section is on the nursing care. Particular focus is on eliciting the specifics of the nursing care of patients with congenital epidermolysis bullosa. Two questions are discussed: 1. What is the specific nursing care of the EB patient? 2. What are the nursing tasks in the treatment of the patient? Fact gathering was carried out with quantitative research, using the process of semi-structured interviews. The first research sample was compiled with three nurses who have had some experience with the nursing care of EB patients. The second research sample was compiled with some mothers whose children suffer from EB. The information gathered was processed in two schematics, consisting of 7 categories and 34 subcategories. The final scientific results led to the conclusion that the nursing care of the EB patient is very individual, with its main requirement being treatment of the damaged skin. The specifics of nursing care are focused on caring and preventing blisters and infections. Other nursing care duties basically include attention to newly formed blisters and injuries due to rubbing and scratching the fragile skin, nutrition, dealing with the patient's mental trauma, patient education to avoid scratching blisters, special communication with the patient, dealing with patient pain, explaining the main points of care during hospitalisation, and a focus on the problems of pseudosyndactyly. EB disease is a very rare, inherited and incurable disease. This Bachelor's thesis should be helpful to nurses, EB patients, parents caring for children with EB, and to the general public as well, with regard to obtaining more information about the disease of congenital epidermolysis bullosa.
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Optical Measurement of Electromechanical Characteristics of Heart Cells
Čmiel, Vratislav ; Križanová,, Olga (referee) ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
Dissertation is focused on the application of optical measurement methods using techniques of optical microscopy and fluorescence microscopy in measurements of electromechanical characteristics of isolated cardiac cells and clusters of differentiated cardiomyocytes. The first proposed method uses a practical combination of fluorescence microscopy equipped with fluorescent fast and high-resolution camera and atomic force microscopy for simultaneous measurement of calcium transients and contraction of cardiomyocyte clusters. The signals obtained undergoes filtration, processing and analysis. Result function parameters obtained by analyzing signals after application of caffeine are evaluated by comparison with functional parameters obtained during the control measurement. The second proposed method is applied to the cardiomyocyte clusters for the purpose of cardiomyocyte contraction signals measurement. The signals obtained by optical methods are analyzed and compared with the reference signal obtained using atomic force microscopy. Optical measurement method of cell contractins based on detection of cell ends using adjusting of microscopy images by re-sharpening and fluorescence method for cardiomyocyte contractions measurements were designed to increase realiability in simultaneous measurement of cell contractions simultaneously with calcium transients in isolated cardiomyocytes experiments.
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