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Hybridizace mezi jelenem evropským a jelenem sikou v oblasti Doupovských hor
Kahajová, Barbora
The sika deer was introduced to the Czech Republic for the first time at the end of the 19th century, after the Second World War it also escaped into the wild. The population numbers of free-living sika deer populations are still increasing and the species causes serious ecological problems. The sika deer is also a major threat to the native European red deer, with which it crossbreeds. This thesis deals with the hybridization between the European red deer and the sika deer. The main goal was to determine the proportion of hybrid individuals between these two species in the area of the Doupovské hory Mountains, where they are in contact. During the hunting season 2020/21, 101 samples of these two species were taken in the study area. I genotyped them using 22 microsatellite loci. Previously genotyped deer samples from this area from the period 2008/09–2016/17 as well as comparative samples of sika deer and red deer from the surrounding areas and samples of the subspecies Dybowski's sika deer from enclosures were added to the evaluated dataset. Using mitochondrial DNA, the samples were divided into individual species or sika deer subspecies. A total of 560 samples were then evaluated using the Bayesian clustering analysis in the program STRUCTURE, of which 270 originated in the Doupovské hory Mountains. Based on the results, 29 hybrids were identified in the dataset, 20 in enclosures, 2 in comparative samples of sika deer and 7 in the study area Doupovské hory Mts. Five of them were detected in the season 2008/09, in the season 2020/21 analyzed by me it was only one individual. The results did not show that the proportion of hybrids in the Doupovské hory Mts has increased significantly, but they confirmed that the cross-breeding of the both species sporadicly occurs there.
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