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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Selekční kritéria k upevnění varroatolerance včely medonosné
 Rod, Jan
Main purpose of this thesis is to summarize knowledge in the field of suppressing the varroosis and to compare contemporary methods and chemicals. Then to describe behavioural patterns of varroatolerant bees, their mutual relations and their importance for selection of varroatolerant honey-bee colonies. In the thesis is described relation between varroosis and the viruses of honey bee and a possible consequences of this relation for the honey bee population. Theoretical knowledge is supported by the experiments with the new methods of application of oxalic acid. The object of these experiments was to determine the efficiency of oxalic acid in control of the growth of the Varroa mite and it’s effect on the brood. Then the varroasensitive hygiene of honey bee colonies was tested. The results show that methods for selecting varroatolerant honey bee colonies is still not finished for praxis, therefore, collapses have to be prevented by application of varroacids.
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Molecular epidemiology of selected viral, bacterial and fungal disease of honeybees in the Czech Republic
 Ryba, Štěpán ; Stopka, Pavel (advisor) ; Voříšek, Karel (referee) ; Půža, Vladimír (referee)
4 Summary Altogether, the six most common bee viruses which infect the honey bee (Apis mellifera) were monitored in the territory of the Czech Republic between 2006 and 2009. Parallel infections of viruses (DWV, ABPV and BQCV) in bee adults and parallel co- infection of viruses with fungal diseases caused by Nosema apis and Nosema ceranae were confirmed by PCR tests. A new sensitive method of detection of the originator of the American foulbrood (Paenibacillus larvae) from bee debris was developed for the practical use of detection of AFB disease in bee populations. Various approaches for the extraction of spores from bee debris and lyses of spores were compared. The sensitivity of PCR tests for the presence of Paenibacillus larvae in debris was compared with the classic cultivation method. The PCR method for the detection American foulbrood was further studied and developed to be more efficient. A new method, based on a matrix-like sample re-arrangement and a use of pooled samples, has been developed for testing 1000 samples in 35 PCR reactions. Another goal was to develop a robust and fast screening method for American foulbrood based on the cultivation test using paper sheets RIDA®COUNT with a specific cultivation medium, specific selection conditions for Paenibacillus larvae and chromogen visualization...
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