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Bioinformatic approaches to the evaluation of frequency population data based on DNA sequence polymorphisms
Milotová, Lujza ; Stenzl, Vlastimil (advisor) ; Daněček, Petr (referee)
This thesis focuses on the problem of efficient management of data useful in DNA analysis, specifically data obtained from short tandem repeats in human DNA, which are of key importance in forensic analysis for the identification of individuals. The thesis presents the design and implementation of a system for the management and visualization of these data from the Czech population, obtained by the Institute of Criminalistics of the Police of the Czech Republic. The main goal was to transform existing data sets into a universal and easy to manage format that would be compatible with existing forensic databases. As a result, the design involves transforming the data into a structured format and then visualising it in tabular layouts, enhancing the ease of data interpretation and manipulation. The thesis also describes the problems identified during data processing and their solutions.
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Detekce variability DNA ve vybrané kolekci ostropestřce mariánského (Silybum marianum L.)
Kratochvílová, Tereza
Milk thistle (Silybum marianum L.) is a promising crop with a wide range of practical applications. It is the only source of the flavonolignan silymarin, which has a positive impact on the health of both humans and animals. Although it is particularly significant as a medicinal plant due to its beneficial effect on liver parenchyma, its genetic diversity has not been thoroughly explored. The study of variability is fundamental for optimizing agricultural production of this crop, clarifying correlations between genome architecture and flavonolignan content, breeding, or categorizing gene sources. This study evaluates the variability of a selected collection of milk thistle using iPBS primers and sequencing analysis of trnC-F and psbA-trnH regions in cpDNA. Knowledge of these sequences can be further utilized, for example, in phylogenetic analyses, diversity studies, and hybridization methods. The study of genetic variability at the molecular level provides important insights for the ideal cultivation or breeding of milk thistle.
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Methodology for detecting the presence of cranberries and lingonberries in processed products using molecular SSR markers: methodology for practice
Kučera, Ladislav ; Ovesná, Jaroslava
The subject of the methodology is a procedure for diagnosing the presence of lingonberries (Vaccinium vitis-idaea L.) and cranberries (Vaccinum macrocarpon L.) in processed products such as tea or fruit ingredients of many food products (jams, jelly, purree, tea). The methodology exploit DNA sequences that are typical for each plant species. Thus, molecular DNA markers that characterize the plant species by SSRs (single sequence repeats) are used. The methodology describes the extraction of DNA from the assumed matrix, the use of selected SSR markers and the analytical data evaluation procedure. The method can be used in any solid equipped molecular-genetic laboratory. The method is applicable for the characterization of DNA isolated from any part of the plant, fruit, and derived food or food supplements. Method describes the procedure and necessary equipment to perform the analysis. Protocol recommends how to evaluate the results.
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