National Repository of Grey Literature 6 records found  Search took 0.01 seconds. 
Virové patogeny hrachu a jejich přenos semenem
Buriánková, Zuzana
This thesis deals with viral pathogens of pea and its seed transmission. It describes characteristic of pea seed-borne mosaic virus (PSbMV) and Pea enation mosaic virus (PEMV), mechanism of seed transmission, methods for detection viruses in seeds and pea resistence to both of viruses. In the experimental part was tested different stages of development of three breeding on new varietes (SM 247, 1019/12 and 1011/12) for presence PSbMV by DAS-ELISA. The aim of the experiment was to determine the developmental stage of pea relevant to determine the transmission PSbMV on pea plants. After the evaluation, it was concluded that this developmental stage is the embryo or one week plant. Furthermore, the selected lines of pea were tested for resistance to PEMV. On the basis of this experiment were obtained pea populations resistant to this virus.
Detection of forage viruses by DAS-ELISA
TOMKOVÁ, Tereza
The subject of this bachelor thesis is screening of viruses on wild and cultivated forage crop growing in the Czech Republic. Two viruses Red clover vein mosaic virus (RCVMV; genus Carlavirus) and Clover yellow mosaic virus (ClYMV; genus Potexvirus) were screened using the DAS-ELISA. Three plants of Trifolium pratense (variety Amos, Blizard and Rezista) were found to be infected with RCVMV, while any plant infected by ClYMV was not identified.
Purification of white clover mosaic virus, antiserum production and IgG isolation for serological detection of virus by ELISA
KOLÁŘOVÁ, Klára
White clover mosaic virus (WClMV) virions were purified using a modified method of Wetter (1960). Antisera were produced by rabbit immunisation and virus-specific polyclonal antibodies were obtained. Suitable conditions were optimised for serological detection of virus by DAS-ELISA and compared with a commercial kit.

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