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Kuřecí embrya jako inkubátor pro pomnožení parazitů rodu Cryptosporidium
SCHULZOVÁ, Tereza
This study aimed to determine the extent to which infection at the embryo stage can enhance the propagation of Cryptosporidium oocysts in chickens. Nine-day-old chicken embryos and one-day-old chickens were experimentally infected with different doses of Cryptosporidium baileyi and Cryptosporidium parvum oocysts. Post hatching, all chickens had demonstrable infections, and the infection dose had no effect on the course of infection. Chickens infected as embryos shed oocysts immediately after hatching and, over the course of the infection, they shed significantly more oocysts than infected oneday-old chickens. In chickens infected as embryos, C. baileyi was found in all organs except the brain, with highest infections in the trachea and oesophagus, and C. parvum was found only in the gastrointestinal tract and trachea, with highest infections in the gut and cloaca. In one-day-old infected chickens, C. baileyi was found only in the gastrointestinal tract and trachea, with highest infections in the gut. Chickens infected as embryos with C. baileyi died within 16 days of hatching. All other chickens cleared the infection within 13-25 days. Based on these findings, infection of chicken embryos could serve as a cost effective and simple model for the propagation of Cryptosporidium spp.
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