|
|
Faktory ovlivňující excystaci žaludečních kryptosporidií přežvýkavců
DUDÁKOVÁ, Hana
In this study, we investigated the effects of temperature and pH on the excystation of gastric Cryptosporidium of mammals. Oocysts of Cryptosporiidum andersoni, obtained from a naturally infected cow, were used in this study. Oocyst viability was de termined by propidium iodide staining (PI). The first factor studied was temperature. Oocysts stored in dH2O were incubated in water bath preheated to temperatures between 32 and 44 °C for 5, 15, and 30 min. With increasing temperature and time, we observed a higher percentage of excysted oocysts. Most oocysts excysted at 40 °C and heating for 30 minutes. In addition, excystation of oocysts was also examined in solutions with different pH values (in a range of 29) heated in a water bath at 40 °C. The optimal pH for excysting is between 6 and 7; however, even at lower values, enough oocysts excyst to cause infection.
|
|
|
Fox cryptosporidiosis
POKORNÝ, Radek
Foxes represent a reservoir of zoonotic diseases and it is necessary perform a control of disease situation. Total 111 fecal samples originated form wild foxes were collected in Czech Republic and Poland in two consequence years 2013 - 2014. All fecal samples were screened for presence of Cryptosporidium spp. using both microscopy and molecular methods. Altogether two samples were molecularly positive and phylogenetic analyses reveal presence C. andersoni in both of them. Low prevalence (1.8%) and low infection intensity was observed in this study. Eighteen cases of diarrhea were detected, but no animal was positive for cryptosporidium infection.
|
|
|
Development of protective immune response in gastric mucosa of mice infected with \kur{Cryptosporidium muris} and \kur{Cryptosporidium andersoni}
JALOVECKÁ, Marie
The development of immune response accountable for the ability to control Cryptosporidium muris TS03 infection was studied using immunocompetent and various types of immunodeficient mouse models. Subsequently the immune response was characterized by analysis of leukocyte infiltration and cytokine production in gastric epithelium. Moreover, the potentiality of immunocompetent mice to develop effective immune response to C. andersoni LI03 infection with consequent protection to consequent infection of the same mice with C. muris TS03 was also studied by monitoring oocysts shedding, leukocyte infiltration of the gastric mucosa and cytokine production in ex vivo cultures of splenocytes.
|
|
|
Prevalence of parasites of alimentary system in young cattle.
ŽIDKOVÁ, Marcela
In biennial following (spring 2005, autumn 2005, spring 2006, autumn 2006) we're rasitology examine 288 samples dropping from heifers and 288 samples dropping from bulls. Exhibits we're examined floatation in Sheather´s sugar solution. In examinate dropping we're proved present cysts Giardia intestinalis and oocysts Cryptosporidium andersoni and family Eimeria. In heifers was most frequent parasites coccidia Eimeria sp. (28,5 %). Flagellata Giardia intestinalis occur in 17 % of all designs. At least we're open up Cryptospordium andersoni (9,4 %). In bulls we're noted highest occurrence coccidia Eimeria sp. (19,1 %). Almost same occurrence we're found out near Giardia intestinalis (18,8 %). Very low prevalence we're have observed near Cryptosporidium andersoni (1,4 %).
|
|
| |
|
|
Infectivity of \kur{Cryptosporidium andersoni} for different species of rodents
ONDRÁČKOVÁ, Zuzana
The infectivity and pathogenicity of Cryptosporidium andersoni (bovine isolate) for neonatal and adult southern multimammate mice (Mastomys coucha) and differently groups of gerbils was studied using transmission experiments. Cryptosporidium andersoni isolate used in this study was not infective for BALB/c mice, but experimental infection proved susceptibility of neonatal and adult M. coucha, neonatal and adult M. unguiculatus, adult Meriones tristrami, Seeketamys calurus, Gerbillus gerbillus to the infection. No signs of clinical illness or macroscopic findings were detected in infected animals. Adult G. gerbillus were the most susceptible to C. andersoni infection. These animals seem to be a useful laboratory model for study of C. andersoni infection.
|
guest ::
