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Určení a kvantifikace mastných kyselin ve vybraných rybích produktech
ONDROUCH, Petr
Determination and quantification of fatty acids in chosen fish products This study is dealing with fatty acids, their nutritional value for human health and the prevention of cardiovascular and other civilization diseases. We introduced catfish Clarias gariepinus biology and importance for the aquaculture and also as a candidate for a valuable source of n-3 PUFAS. The chemical-analytical approach was used to determine content of fatty acids in chosen meat products from mentioned extraordinary fish. Extraction of lipids, derivatization of fatty acids and gas chromatography (GC FID) were performed. Furthermore, the meat products from the Clarias gariepinus were compared with classical pork products - sausages. The results showed that fish products seem to be a valuable source of docosahexaenoic and eicosapentaenoic acid, the "classical" sausages, on the contrary, contain none of the desired fatty acids. Additionally, the fish products are designed as sausages and therefore resemble the "classical" ones which could be attractive even for the conservative consumers. Consumption of fish products will lead to elevation of daily intake of n-3 PUFAs and thus positively influence the common health by preventing cardiovascular and other civilisation diseases.
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Impact of teperature, fish size and feeding on oxygen consumption and ammonia excretion in the african catfish (Clarias gariepinus)
KOMENDOVÁ, Jana
Aims of this thesis were to assesed the impact of feeding, temperature of water and fish size on ammonia excretion and oxygen consumption in Clarias gariepinus in recirculating system. Fish were divided in 8 weight categories from 21 to 2495 g. Experiments were performed under four temperatures: 22°C, 25°C, 28°C and 30°C. Fish were acclimatized, light conditions were: 14 hours of light period and 10 hours of dark period. Feeding was provided fed four times a day. Starved fish were measured. Measurements were held every two hours. Oxygen consumption was measured by multimeter and ammonia excretion using Nessler?s method. Average daily oxygen consumption in fed fish was dependent on temperature and average individual weight of fish, varied from 10.5 to 96.6 mg O2/kg/h and in hungry fish from 4.3 to 61.8 mg O2/kg/h. TAN excretion varied from 0 to 59.9 mg TAN/kg/h in fed fish and from 0 to 60.8 mg TAN/kg/h in hungry fish. Oxygen consumption increased with increasing temperature. Ammonia excretion was very unstable at all temperatures in all weight categories. Fish had higher ammonia excretion in light period of experiment.
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