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National Repository of Grey Literature 2 records found  Search took 0.02 seconds. 
Application of fluorescence staining for the "Scratch Wound Healing Assay" experiment
 Zumberg, Inna ; Svoboda, Ondřej (referee) ; Chmelíková, Larisa (advisor)
This bachelor’s thesis studies the cell culture of 3T3 cell line (mouse embryonic fibroblast cells) and cell migration. The Scratch Wound Healing Assay was used for the study of cell migration. The basic principles of experimenting, its advantages and disadvantages, as well as the practical use of this method in scientific research were presented on specific examples. Practical part of the bachelor’s thesis describes the experiment in the cell laboratory including cell culture of 3T3 cell line. The proposed experiment was tested with a sufficient number of repetitions. The statistical processing of microscopic images was performed in the MATLAB programming environment.
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Application of fluorescence staining for the "Scratch Wound Healing Assay" experiment
 Zumberg, Inna ; Svoboda, Ondřej (referee) ; Chmelíková, Larisa (advisor)
This bachelor’s thesis studies the cell culture of 3T3 cell line (mouse embryonic fibroblast cells) and cell migration. The Scratch Wound Healing Assay was used for the study of cell migration. The basic principles of experimenting, its advantages and disadvantages, as well as the practical use of this method in scientific research were presented on specific examples. Practical part of the bachelor’s thesis describes the experiment in the cell laboratory including cell culture of 3T3 cell line. The proposed experiment was tested with a sufficient number of repetitions. The statistical processing of microscopic images was performed in the MATLAB programming environment.
Detailed record

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