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Analysis of NGS data for study of transposon activity in cancer cells
Hrazdilová, Ivana ; Čegan,, Radim (referee) ; Eduard, Kejnovský (advisor)
Theoretical part of this diploma thesis gives a brief characteristic of human mobile elements (transposons), which represents nearly 50% of human genome. It provides basic transposon clasification and describes types of transposons present in hunam genome, as well as mobilization, activation and regulation mechanisms. The work also deals with the domestication of transposons, describes the ways in which TE contribute to DNA damage and summarizes the diseases caused by mutagenic activity of transposons in the human genome. Conclusion of theoretical part describes next-generation sequencing technologies (NGS). As practical part, data from RNA-seq experimet were analyzed in order to compare differen transposon activity in normal and cancer cells from prostate and colorectal tissues. As like as publicly available sophisticated tools (TopHat), new scripts were created to analyze these data. The results show that cancer cells exhibit overexpression of transposons. This corresponds with the published results and suggests a connection of transposon activation with cancer development.
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Risk Management Options for Testing Laboratories in the Context of Revised Standards of Management Systems
Suchý, Ivo ; Misák, Petr (referee) ; Vymazal, Tomáš (advisor)
The diploma thesis deals with analysis and assessment of risks in testing laboratories, where the work with risks is an unrecognized area. A part of the thesis defines risks according to standard revision 17025:2017. Subsequently, these risks are processed by selected methods, which are used for qualitative and quantitative evaluation. Risks determination in the thesis is based on methods Risk Matrix, Failure Modes & Effects Analysis (FMEA) and Bow Tie Analysis. Introducing possibilities and patterns of described methods to testing laboratories is the main aim of the thesis, this could be a way to increase their chance to get a new accreditation.
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Risk Management Options for Testing Laboratories in the Context of Revised Standards of Management Systems
Suchý, Ivo ; Misák, Petr (referee) ; Vymazal, Tomáš (advisor)
The diploma thesis deals with analysis and assessment of risks in testing laboratories, where the work with risks is an unrecognized area. A part of the thesis defines risks according to standard revision 17025:2017. Subsequently, these risks are processed by selected methods, which are used for qualitative and quantitative evaluation. Risks determination in the thesis is based on methods Risk Matrix, Failure Modes & Effects Analysis (FMEA) and Bow Tie Analysis. Introducing possibilities and patterns of described methods to testing laboratories is the main aim of the thesis, this could be a way to increase their chance to get a new accreditation.
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Analysis of NGS data for study of transposon activity in cancer cells
Hrazdilová, Ivana ; Čegan,, Radim (referee) ; Eduard, Kejnovský (advisor)
Theoretical part of this diploma thesis gives a brief characteristic of human mobile elements (transposons), which represents nearly 50% of human genome. It provides basic transposon clasification and describes types of transposons present in hunam genome, as well as mobilization, activation and regulation mechanisms. The work also deals with the domestication of transposons, describes the ways in which TE contribute to DNA damage and summarizes the diseases caused by mutagenic activity of transposons in the human genome. Conclusion of theoretical part describes next-generation sequencing technologies (NGS). As practical part, data from RNA-seq experimet were analyzed in order to compare differen transposon activity in normal and cancer cells from prostate and colorectal tissues. As like as publicly available sophisticated tools (TopHat), new scripts were created to analyze these data. The results show that cancer cells exhibit overexpression of transposons. This corresponds with the published results and suggests a connection of transposon activation with cancer development.
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