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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Laboratory diagnostics of Lyme disease
 DOSTÁLOVÁ, Simona
Lyme borreliosis is the most common infectious disease that is transmitted by ticks infected with Borrelia burgdorferi bacteria (anthropozoonosis). The diagnosis of this disease is based on the detection of specific IgG and IgM class immunoglobulins by specific laboratory tests. This bachelor thesis focuses on the evaluation of the clinical condition of patients by the attending physician (see case reports) in accordance with the results of laboratory tests. The correct diagnosis is based on the clinical symptoms, anamnesis and laboratory findings of each patient, which is used to guide adequate antibiotic treatment. In this work a comparison of two methods used for detection of antibodies against Borrelia burgdorferi, the chemiluminescence immunoassay (CLIA) method and the confirmatory Western blotting method used in the Clinical Laboratory of DIA-GON MP, s.r.o. in Cheb, is performed. The CLIA method provides rapid and sensitive screening for IgG and IgM antibodies, while Western blotting serves as a confirmatory test with higher specificity. The evaluation of the results and their interpretation are based on the detection of specific antibodies against Borrelia by Western blotting. The last part of the bachelor thesis is a search of an article from the Czech Hydrometeorological Institute, which cooperates with the State Institute of Health with the support of the Ministry of Health of the Czech Republic. It discusses tick activity in relation to their seasonal occurrence. On the basis of this article, I make an assessment of the seasonal occurrence of Lyme borreliosis in 2015-2017 with respect to the average temperatures in that period.
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