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Bloodstream infection in patients during the COVID-19 pandemic in of General University Hospital in Prague
BAUER, Kristýna
Blood stream infection is one of the serious life-threatening conditions of hospitalized patients with coronavirus disease during the COVID-19 pandemic in the Czech Republic. This is the penetration of microorganisms into the bloodstream, which is accompanied by symptoms of a general infection. The most common consequence is the development of sepsis. The aim of the laboratory examination is to obtain a reliable blood culture result. Such a result can only be achieved if all procedures are followed, from the indication by the doctor to the process of sampling by the medical staff into the bottles directly intended for this purpose. Taking blood cultures confirms or excludes the presence of bacteria in the bloodstream. Blood cultures are the gold standard for diagnosing bloodstream infections. A detection culture is used for hemocultivation of the BacT/Alert 3D system, which is based on the colorimetric detection of CO2 produced by growing microorganisms in blood cultures.
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Septic condition from the perspective of clinical microbiology
SZITAIOVÁ, Markéta
This thesis focused on the issue of bloodstream infections from the poin of view of clinical microbiology. Diagnostics using BACT/ALERT Virtuo automatic blood culture system. In the theoretical part of the thesis, there are mentions of diseases of the blood stream, the causative agents of sepsis, and the method of isolation. In the practical part of the thesis, data obtained in the Pilsen University Hospital are processed, focusing on the most common agents causing sepsis.
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Nursing care of vascular access focused to prevention of catheter sepsis
Prošková, Michaela ; Heczková, Jana (advisor) ; Chvojková, Kateřina (referee)
Ensuring the bloodstream is one of the most frequently indicated invasive procedures, especially in the pre-hospital emergency and intensive care. All invasive inputs are nowadays an integral part of intensive care. Despite careful daily care, vascular accesses are at risk of complications, which annually effects many patients institutionalized at intensive care units. Catheter sepsis is undoubtedly one of the most serious. Although a significant proportion of these infections are considered highly preventable, they still contribute to high morbidity and mortality among these patients. The aim of this diploma thesis is to analyse nursing care for vascular inputs. One of the partial goals is to evaluate and compare the relevance of the nursing standard of a medical facility with scientific knowledge and current recommendations of professional societies. The research method is a quantitative questionnaire survey focused on nursing staff working in the Department of Anesthesiology and Reanimation. The analysis of the vascular inputs re-dressings was performed in patients hospitalized in the same ward, where the research itself took place. The results of the research survey show a discrepancy between the given procedure in the nursing standard and common practice in the ward. In view of the...
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Bacteriological examination of blood cultures
MAJEROVÁ, Natálie
The aim of this bachelor thesis is the introduction of possibilities of hemoculture examination, assuming the methodology of evolving the manual and automated hemocultures in tha Stafila laboratories and at the Department of Bacteriology in the Hospital of České Budějovice. Then it deals with the comparison of those two systems and learning the basic interpretation of results. In the theoretical part I focus on blood circulation infections, sepsis and its diagnostics and nozocomial infections.The last part delas with the most frequent bacterial causers of blood circulation infections. The practical part focuses on the approach of hemoculture process, firstly on the preanalytic part which includes the sample collection and its transport. Secondly there is the analytic part where cultivation bottles are described as well as the basic methods for bacteria certification such as making the microscopic sample and bacteria cultivation. The next part delas with the determination of a bacteria kind and its antibiotics sensitivity.In the last part there are results of cultivation of the followed up file from the both methods. Positive findig occuresd in 15% of taken hemocultures.
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