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Evaluation of genetic strains polymorphism of \kur{Beauveria bassiana} based on analyses of using microsatellite markers
PUDIL, Jiří
Microsatellites, or Simple Sequence Repeats (SSRs), are highly polymorphic loci present in nuclear and organellar DNA that consist of repeating units of 1-6 base pairs in length. Microsatellites are very effective molecular markers, which allow us to examine various aspects of organisms. The aim of thesis is to carry out analyzes of microsatellites and describe the genetic relationships between individual strains and monosporic isolates, which were derived from these strains. Usind SSR I would like to asses the degree of genetic polymorphism between \kur{Beauveria bassiana} strains, collected during monitoring in the locality of Černá Hora in Šumava. I am also trying to optimize the method of DNA isolation and method of microsatellites analysis. 44 strains of \kur{Beauveria bassiana} isolated from \kur{Ips typographus} were analyzed in total using four primers. Overall, the results that I obtained show high similarity of evaluated strains, and therefore low level of genetic polymorphism. For further work it would be appropriate to enlarge the area, which will be sampled and include sets of strains that were obtained from different developmental stages of the host, as well as other reference strains of entomopathogeníc fungi collected from other localities with similar conditions. For more detailed study it would be useful to use the analysis of ribosomal DNA. Thus selected and evaluated population is likely to provide a more veritable image of strain diversity in observed area.
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Use of entomopathogenic fungi in biological control against sweetpotato whitefly Bemisia tabaci
ŘEHOŘOVÁ, Markéta
The thesis is analyzing effects of selected species and strains of entomopathogenic fungi on the synchronized population of Bemisia tabaci under optimal and sub-optimal conditions. The following species of fungi were used during the experiments: Aschersonia aleyrodis, Lecanicillium lecanii (former Verticillium lecanii), Isaria fumosorosea (former Paecilomyces fumosoroseus) a Beauveria bassiana. There was a experiment of mortality of all these fungi in the frequency of 7, 14, and 21 days, while all these experiments were made under the same conditions (concerning temperature and humidity) in order to keep all the results comparable. In the case of Isaria fumosorosea, the experiment was taken also under sub-optimal conditions in order to compare effectiveness of this significant fungi, both under the optimal conditions (relative air humidity 95-100 %, temperature 25{$\pm$}1°C), and sub-optimal conditons (relative air humidity bellow 75 %, temperature 25{$\pm$}1°C). Greater attention was also given to A. aleyrodis which represents one of the most important fungi on the field of whitefly combat.
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