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The basic microbiological examination of blood cultures in the automated blood culture system
ŽÁKOVÁ, Hana
This bachelor thesis is focused on examination of blood cultures in automatic blood culture system. In the theoretical part, it deals mainly with general terms of the presence of bacteria in the blood and the development of sepsis, as well as with the description of the most frequent infectious agents in blood cultures in different groups of patients and also with different types from the oldest to newest blood culture systems. Manual systems are described, such as Septi-Chek and Oxoid Signal System, and automated BACTEC and Bact / Alert systems that work on different principles. Also discussed is the subsequent processing of samples to identify microbial agents and determine their susceptibility to antibiotics. The aim was to acquire practical processing of blood cultures in automatic BACTEC hemocultivation system. The methodical part is devoted to the preparation and processing of samples for hemoculture, the subsequent procedure of inoculation on the cultivation soil and the preparation of a microscopic preparation stained by Gram. Furthermore, the thesis deals with the statistical processing of data provided at the microbiological department of the Nemocnice Písek, a.s. The statistical survey was carried out in order to determine the microbial spectrum in blood cultures and the percentage of positivity to the total number of collected blood cultures in 2014-2018. A total of 9136 blood cultures from 3027 patients were examined during the study period. Of the total number, 1663 were positive, i.e. 18 %. 82 % of blood cultures were negative, did not show growth of microorganisms. The microbial spectrum is very wide, with Escherichia coli, Staphylococcus aureus and Klebsiella species.
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The basic microbiological examination of blood cultures in the automated blood culture system
ŽÁKOVÁ, Hana
This bachelor thesis is focused on examination of blood cultures in automatic blood culture system. In the theoretical part, it deals mainly with general terms of the presence of bacteria in the blood and the development of sepsis, as well with the description of the most frequent infectious agents in blood cultures in different groups of patients and also with different types from the oldest to newest blood culture systems. Manual systems are described, such as Septi-Chek and Oxoid Signal System, and automated BACTEC and Bact / Alert systems that work on different principles. The subsequent processing of samples is also discussed to identify microbial agents and determine their susceptibility to antibiotics. The aim was to acquire practical processing of blood cultures in automatic BACTEC hemocultivation system. The methodical part is devoted to the preparation and processing of samples for hemoculture, the subsequent procedure of inoculation on the cultivation soil and the preparation of a microscopic preparation stained by Gram. Furthermore, the thesis deals with the statistical processing of data provided at the microbiological department of the Nemocnice Písek, a.s. The statistical survey was carried out in order to determine the microbial spectrum in blood cultures and the percentage of positivity to the total number of collected blood cultures in 2014-2018. A total of 9838 blood cultures were examined during the study period. Of the total number, 2338 were positive. That is 24%. 76% of blood cultures were negative, did not show growth of microorganisms. The microbial spectrum is very wide, the most common infection agents are Staphylococcus plasmakoagulasa negative, Escherichia coli, Staphylococcus aureus subsp. aureus, Staphylococcus epidermidis and Klebsiella species.
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