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Catheter-based left atrial appendage closure
Hozman, Marek ; Osmančík, Pavel (advisor) ; Bulava, Alan (referee) ; Havránek, Štěpán (referee)
Transseptal puncture in left atrial appendage closure guided by 3D printing and multiplanar CT reconstruction Abstract Introduction: The presented study investigates the application of bi-atrial 3D printed models to guide transseptal puncture (TSP) in left atrial appendage occlusion (LAAC). The objectives are to (1) test the feasibility of 3D printing (3DP) for TSP guidance, (2) analyse the distribution of the optimal TSP locations, and (3) define a CT-derived 2D parameter suitable for predicting the optimal TSP locations. Methods: Pre-procedural planning included multiplanar CT reconstruction, 3D segmentation, and 3DP. TSP was pre-procedurally simulated in vitro at six defined sites. Based on the position of the sheath, TSP sites were classified as optimal, sub-optimal, or non-optimal. The aim was to target the TSP in the recommended position during the procedure. Procedure progress was assessed post hoc by the operator. Results: Of 68 screened patients, 60 patients in five centres (mean age of 74.68 ± 7.64 years, 71.66% males) were prospectively analysed (3DP failed in one case, and seven patients did not finally undergo the procedure). In 55 patients (91.66%), TSP was performed in the optimal location as recommended by the 3DP. The optimal locations for TSP were postero-inferior in 45.3%, mid-inferior in...
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Voltammetric Determination of Warfarin at a Screen-Printed Carbon Electrode
Žužičová, Victória ; Vyskočil, Vlastimil (advisor) ; Dejmková, Hana (referee)
In this Bachelor Thesis, the electrochemical behaviour of the drug warfarin sodium was studied at a screen-printed carbon electrode (SPCE) using DC voltammetry (DCV) and differential pulse voltammetry (DPV). The dependence of the voltammetric behaviour of the substance on the pH of the medium was studied in Britton-Robinson buffer (BR buffer) in the pH range of 2.0−12.0. A pH of 7.0 was chosen as the optimum pH for both voltammetric techniques. A repeatability of 3.8% for DCV and 4.3% for DPV was determined for the selected pH. Calibration dependence was monitored under the selected optimal conditions in the concentration range 2∙10−6 to 1∙10−4 mol∙dm−3 . The limit of detection (LOD) was determined to be 1.05∙10−6 mol∙dm−3 for DCV and 2.59∙10−7 mol∙dm−3 for DPV. The limit of quantification (LOQ) was 3.50∙10−6 mol∙dm−3 for DCV and 8.62∙10−7 mol∙dm−3 for DPV. These newly developed methods were used for the determination of the active ingredient in the pharmaceutical formulation Warfarin Orion 5 mg. The values measured by voltammetric methods were compared with those obtained by spectrophotometric measurements. Key words Electrochemistry, Warfarin, Anticoagulants, Direct Current Voltammetry, Differential Pulse Voltammetry, Screen-Printed Carbon Electrode
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