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Regenerace smyslových orgánů u raků
KOR, Golara
The antennae are a crucial sensory organ in crayfish that plays vital roles in their survival, communication, and orientation within their environment. They are equipped with sensory hairs that enable crayfish to detect various stimuli. Crayfish possess the unique ability to regenerate their antennae. Hemocytes are specialized cells within crayfish and play a crucial role in their immune system and contribute to the process of regeneration. We applied cryo-scanning and Transmission electron microscopies to study sensory organ morphology and regeneration in crayfish. In Chapter 2, cryo-scanning electron microscopy was used to identify potential differences in antennal morphology in six different crayfish species including marbled crayfish Procambarus virginalis, Mexican dwarf crayfish Cambarellus patzcuarensis, red swamp crayfish Procambarus clarkii, signal crayfish Pacifastacus leniusculus, common yabby Cherax destructor, and spiny-cheek crayfish Faxonius limosus. We observed significant differences among the six crayfish species in the ratios of antenna length, segment length and width to carapace length, and the number of segments. Examination of ultrastructural features unveiled differences in the distribution patterns of sensory hairs along the antenna and the morphology of the antennal surface. The varying morphology of antennae among studied species possibly indicates an adaptation to the specific conditions of their respective habitats. Furthermore, the outcomes demonstrated that a combination of variations in both antennal morphological characteristics and biometric measurements could effectively help us to distinguish the different studied crayfish species. In Chapter 3, the ultrastructural behaviour of hemocytes during coagulation and phagocytosis in the initial phases of injury in marbled crayfish was explored using transmission electron microscopy. During the coagulation process, hemocytes experienced marked transformations in morphology. The cytoplasmic granules exhibited a change from electron-dense to electron-lucent forms with the progress of coagulation. The transformed granules containing amorphous, electron-lucent material were observed to combine and release their contents into the extracellular space as part of the coagulation process. Additionally, it was observed that the nucleus contents also play a role in the coagulation process. Furthermore, the amputation of the leg led to substantial muscle degeneration, and phagocytic hemocytes start to take up the necrotic tissues. Besides, we observed the digested remains from phagocytized necrotic tissues merged into granules and other cellular components, thereby enhancing the granularity of the hemocytes and altering their cellular morphology. However, it is essential to note that hemocyte degranulation during coagulation could potentially reduce their granularity. Since morphological features are critical for classifying hemocytes, these morphological changes during coagulation and phagocytosis must be considered significant factors. The degenerated material that stored inside phagocytic hemocytes may have application in immunological and regeneration processes. In Chapter 4, we employed transmission electron microscopy to explore the ultrastructural aspects of potential immune cell involvement in nerve regeneration within crayfish antennae post-amputation. The findings indicated that, during nerve regeneration, all three forms of hemocytes were present. However, the granules within semi-granulocytes and granulocytes mainly contributed to the generation of new organelles such as mitochondria, the Golgi apparatus, and nerve fibers in the regenerated nerves of crayfish antennae. We revealed the transformation of granules of hemocytes into diverse organelles during the process of nerve regeneration at the ultrastructural level. In conclusion, these granules function as compact repositories of adaptable materials carried by immune cells, capable of transformatio
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Suggestion of changes of infrastructure of network for RAAB Computer
Brázda, Ondřej ; Raab, Ladislav (referee) ; Ondrák, Viktor (advisor)
The target of this work is focused on relocation of important backbone node of network and implementation of internet signal to villages that are accessible with difficulties. The reason why the backbone node has to be relocated is because the building where the backbone node is located will be demolished. On the basis of this thesis the company should be able to realize changes in its network. In the theoretical part of this thesis I am going to deal with possible problems in transmission of internet signal.
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RFID Reader for 13.56 MHz Band
Obšitník, Michal ; Zamazal, Michal (referee) ; Povalač, Aleš (advisor)
Cieľom tejto práce je navrhnúť RFID čítačku pre pásmo 13.56 MHz a zostaviť k nej riadiaci program. V prvej časti je najskôr práca smerovaná k oboznámeniu sa s princípom fungovania technológií RFID a NFC, spolu s tým súvisiacimi medzinárodnými štandardami a k oboznámeniu sa s rôznymi dostupnými čipmi na obsluhu týchto technológií. Keďže čip s ktorým sa bude pokračovať je jasne zadaný v návode, ich porovnanie slúži čisto oboznamovaciemu účelu. V praktickej časti je úloha navrhnúť a oživiť prototyp RFID čítačky. Základom je doska sprostredkúvajúca RFID komunikáciu, audiovizuálnu odozvu a pripojenie k OLED displeju. Táto doska je pripojená na mikrokontrolér na ktorý riadi celé ovládanie zariadenia. Súčasťou projektu je aj set rôznych antén, ktoré je možné na dosku pripojiť a zároveň ich vymieňať. Vyrobené antény sú podrobené testovaniu ako dve rozdielne metódy ladenia ovplyvnia ich chod. Testujú sa maximálna vzdialenosť čítania, úspech prevedených čítaní a hodnota prúdu, ktorá sa vznesie pri kalibračnom procese. Výstupom práce je RFID čítač s riadiacim programom.
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Construction of an impedance analyzer
Slinták, Vlastimil ; Smékal, Zdeněk (referee) ; Mikulka, Jan (advisor)
The aim of this master’s thesis is to build stand-alone impedance analyzer for mesasuring antennas’ impedance. The vector network analysis is used as measuring method. Analog (with directional coupler and gain and phase detector) and digital (with 8bit AVR microcontrollers) part of analyzer are described and then build.
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Migration of telemetric narrowband radio networks to the IP standard
Stupka, Richard ; Lattenberg, Ivo (referee) ; Maršálek, Roman (advisor)
This bachelor’s thesis deals with the innovation of narrowband telemetry radio networks to the IP standard. This involves the modernization of old radio data networks that do not use the IP standard or are nearing the end of their life cycle. Renovation has a great benefit in terms of performance, reliability, and security. This modernization is achieved through migration, which means upgrading the old technologies to new ones. The objective of this thesis was to develop theoretical analysis and a technical procedure for network migration. It was deals with the currently used technologies and utilized components, parameters of individual network nodes, the actual design of the migration technical solution and network preparation. After the analysis and technical solution for migration had been performed, the proposed migration solution through simulated operation in a laboratory environment was verified. Based on the obtained information, configuration files for real-world usage were created, radio signal coverage in the field was verified and the communication capability in the newly built RipEx2 network was tested.
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Mobile station for tracking the position of satellites
Zdražil, Jakub ; Dvořák, Radim (referee) ; Mikulášek, Michal (advisor)
This bachelor's thesis deals with the creation of a mobile device that enables to automate the tracking of satellite positions based on data downloaded from the internet. The thesis consists of a theoretical part, where the reader learns about the history of satellite systems, their types, various orbit types, and their characteristics. It also includes information about the behavior of different types of antennas and reasons for selecting an antenna suitable for this project. The last chapter of the theoretical part consists of available communication IoT technologies and the selection of a suitable technology for this project. The practical part includes the design of a PCB board, the design of the device's body, and the design of software support. The conclusion summarizes the insights gained from the device production and any potential changes for future iterations of the device.
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RFID Reader for 13.56 MHz Band
Obšitník, Michal ; Zamazal, Michal (referee) ; Povalač, Aleš (advisor)
Cieľom tejto práce je navrhnúť RFID čítačku pre pásmo 13.56 MHz a zostaviť k nej riadiaci program. V prvej časti je najskôr práca smerovaná k oboznámeniu sa s princípom fungovania technológií RFID a NFC, spolu s tým súvisiacimi medzinárodnými štandardami a k oboznámeniu sa s rôznymi dostupnými čipmi na obsluhu týchto technológií. Keďže čip s ktorým sa bude pokračovať je jasne zadaný v návode, ich porovnanie slúži čisto oboznamovaciemu účelu. V praktickej časti je úloha navrhnúť a oživiť prototyp RFID čítačky. Základom je doska sprostredkúvajúca RFID komunikáciu, audiovizuálnu odozvu a pripojenie k OLED displeju. Táto doska je pripojená na mikrokontrolér na ktorý riadi celé ovládanie zariadenia. Súčasťou projektu je aj set rôznych antén, ktoré je možné na dosku pripojiť a zároveň ich vymieňať. Vyrobené antény sú podrobené testovaniu ako dve rozdielne metódy ladenia ovplyvnia ich chod. Testujú sa maximálna vzdialenosť čítania, úspech prevedených čítaní a hodnota prúdu, ktorá sa vznesie pri kalibračnom procese. Výstupom práce je RFID čítač s riadiacim programom.
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Suggestion of changes of infrastructure of network for RAAB Computer
Brázda, Ondřej ; Raab, Ladislav (referee) ; Ondrák, Viktor (advisor)
The target of this work is focused on relocation of important backbone node of network and implementation of internet signal to villages that are accessible with difficulties. The reason why the backbone node has to be relocated is because the building where the backbone node is located will be demolished. On the basis of this thesis the company should be able to realize changes in its network. In the theoretical part of this thesis I am going to deal with possible problems in transmission of internet signal.
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Construction of an impedance analyzer
Slinták, Vlastimil ; Smékal, Zdeněk (referee) ; Mikulka, Jan (advisor)
The aim of this master’s thesis is to build stand-alone impedance analyzer for mesasuring antennas’ impedance. The vector network analysis is used as measuring method. Analog (with directional coupler and gain and phase detector) and digital (with 8bit AVR microcontrollers) part of analyzer are described and then build.
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