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Tapeworm parasitic in the digestive tract rays Amblyraja radiata on Svalbard
PADALÍKOVÁ, Petra
This bachelor thesis is interested in tapeworms of the stingray Amblyraja radiata. These rays Ambyraja radiata living in Svalbard. It is known about 5000 species of parasitic tapeworms which are parasites on vertebrates. The highest number of tapeworms lives in cartilaginous fish, and fish. Adults are found in the digestive system of vertebrates. In obtained samples were found three genera of tapeworms: Grillotia, Pseudanthobothrium, Echeneneibothrium. My objective was to determine the species. My aim was to compare the results with the literature and characterize the community tapeworm rays Amblyraja radiata in Svalbard. Identification of tapeworms was based on morphological characters. Morphological features are (eg. strobili maximum width, length cirrus sac, testes number and shape of hooks.) These characters I visualized by means of staining, light microscopy and electron microscopy. I used a carmine staining into individual samples tapeworms. Thanks to this coloring I achieved better visibility of internal organs, particularly genitals. Thanks to scanning electron microscopy I was allowed to observe the specimen surface.
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Blood parasites of ray from Svalbard
JAKEŠOVÁ, Anežka
This Bachelor's thesis is about blood parasites of fish and chondrichthyes. In this case especially thorny skate Amblyraja radiata. Blood smears of fish and chondrichthyes for this thesis were obtained from Svalbard, which I deal with at the beginning. In the theoretical part, the division of the chondrichthyes as well as parasitism in general are descibed. In the followinf description I mentioned blood parasites, trematodes and nematodes, which can also appear in the blood of the vertebrates. The blood parasites aslo includes Haemogregarina delagei, which is the only parasite found on blood smears of thorny skate Amblyraja radiata and Desseria myoxocephali on fish Myoxocephalus scorpius. A. radiata are infected by H.delagei in high prevalence (90 %) on Svalbard. In the practical part, I have dealt with all the steps to gain part of the gene 18S rRNA. Isolated DNA from ray blood was added in several different mixtures PCR products, which were different by polymerase buffer or primers. I obtained the size of the PCR products by agarose electrophoresis, isolated by commercial type (Expin Combo, GeneAll) and sent to the Seqme company for a direct sequencing, or I used it for molecular cloning. I isolated well-cloned DNA segments from competent cells by using a kit (Hybrid-Q Plasmid, GeneAll) and sequenced in the Seqme service company again.Obtained sequencies are similar to Desseria myoxocephali, and it reflects the hypothesis, that Haemogregarina is not monophyletic genus.
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