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Využití přípravků umožňujících kontrolované uvolňování hormonální látky v umělé reprodukci ryb
PECHA, Oldřich
This bachelor thesis was focused on identification of species-optimized preparations based on PLGA microparticles in terms of length and intensity of release and composition of active substance (GnRHa, metoclopramide), which would be used in artificial reproduction of selected fish species - pike (Esox lucius). Under the conditions of the Experimental Fish Farm, FROV JČU in Vodňany and the Tábor Štičí líheň ESOX s.r.o. the first two-week experiment was conducted to achieve the desired results, which were achieved with males of pike. A year later, the experiment took place again in Tabor and newly in the hatchery in Mydlovary. This time we focused on females of pike. During the first research, we have achieved satisfactory results and confirmed that we are going in the right direction. Combination of GnRH with dopamine antagonist Metoclopramide has been shown to be most effective. The best results were achieved by application of 20?g GnRHa.kg-1 + 20mg MET.kg-1, where we achieved positive results in all parameters - increased milt production volume, increased sperm motility and speed, decreased 11-ketotestosterone concentration. During the second experiment with female, we showed that the combination of GnRH + MET at a concentration of 20?g GnRHa.kg-1 + 20mg MET.kg-1 has a positive effect on ovulation achievement, latency time reduction and higher concentration of 40?g GnRHa.kg-1 + 20 mg MET.kg-1 also for fertilization and hatching of eggs, but at the cost of lower fecundity. The results of the experiments show that by using this combination and the correct concentration of GnRH with the dopamine antagonist it is possible to induce maturation of the gametes of male as well as female of pike fish. However, there is still room for improvement of female.
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Odběr spermatu pomocí katetru a jeho využití při výtěru štiky obecné (Esox lucius L.)
PLAŇANSKÝ, Tomáš
The aim of this diploma thesis is to compare quality of northern pike sperm collected by different methods. First method is collection of stripped sperm by abdominal massage of the belly. Second method is collection of stripped sperm with special catheter to eliminate sperm contamination by urine. The last method is collection of testicular sperm. Differently collected sperm was evaluated and compared its quality. The main observed parameters were sperm volume, spermatozoa concentration, spermatozoa motility and velocity and osmolality of seminal fluid. Sperm samples were used for eggs fertilization. In fertilized eggs, the fertility of eggs and larvae hatching rate were observed.
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Vývoj úlovků ryb v údolní nádrži Dalešice na řece Jihlavě
Gregr, Tomáš
The Bachelor thesis focuses on evaluation of caught trend in dam reservoir Dalešice (fishery Jihlava 7-8). The main object aim is to evaluate fish caught by angling in decade 2005 - 2014. Thesis focuses on predatory fish species and their ratio to other species. There is used research from 1991 - 2000 to compare results. This work discuses dam reservoirs and species structure of the fish community, compares each species of fish between them and its suitability for dam reservoirs. Amount of predatory fishes caught by anglers decreases during evaluated ten years, depending on quantity of each predatory fish species. The biggest influence had pike (Esox lucius), pikeperch (Sander luciopeca) and asp (Leuciscus aspius).The ratio between predatory fishes and other fish species depends also on proportion of each fish species to total richness of predatory fish species. In the second half of decade (since 2010) the ratio increases in fa-vour of not predatory (cyprinid) fish species.
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Optimalization of artificial incubation of eggs and embryos in northern pike (\kur{Esox lucius L.}) under controlled conditions
HAMPL, Jan
Within my diploma thesis I have been trying to resolve the possibilities of optimization of artificial incubation of eggs and embryos in northern pike (Esox Lucius L.) under controlled conditions during three different experiments. In the first experiment was investigated the effect of three different semination solutions to the success of incubation of eggs and embryos in northern pike. I watched the survival of eggs and embryos, larvae hatching abnormalities and biometrics of larvae. It was found that the best overall results were achieved with semination solution composed of 15 ml of chemical crystalic urea per 1 liter of water, with an appropriate amount of abnormalities and highest larvae survival. In the second experiment were performed manipulation with developing eggs at given time intervals after fertilization of eggs. I watched the survival of eggs and embryos, abnormality and biometrics of hatched larvae. It was found that the lowest survival, abnormalities, and smallest TL and W were reached during manipulations within 24 hours after fertilization. In the third experiment was investigated the effect of water temperature on the length of the incubation of eggs and embryos in northern pike. With regard to the overall survival, larvae hatching, abnormalities and size of larvae achieved the best results temperatures 6 and 10 ° C. Conversely, it is entirely unsuitable for incubation temperature of 3 ° C (lowest survival, large amounts deformities). Different incubation temperatures have also confirmed the effect of water temperature on biometrics of hatched larvae.
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Reprodukce a intenzivní chov juvenilních ryb štiky obecné (Esox lucius L.)
BONDARENKO, Volodymyr
Fish hatcheries do not always provide optimal conditions for controlled reproduction and production of high-quality juvenile in northern pike. This study was undertaken to optimize reproduction and intensive juvenile culture of northern pike (Esox lucius L.) under controlled conditions. The efficacy of traditional treatments for induction and synchronization of ovulation in northern pike (carp pituitary or ambient outdoor conditions) was compared to that of sGnRH-a at 50 and 100 ?g kg-1 with or without Freund´s incomplete adjuvant and dopamine inhibitor (metoclopramide, 8 mg kg-1). Ovulation was observed in females treated with carp pituitary (3 mg kg-1) and in those held in ambient conditions. Sperm motility parameters were compared in northern pike and sterlet (Acipenser ruthenus Brandt). Analysis was accomplished via high speed video microscopy complemented with CASA analysis. This work described recently developed methods of recognizing and quantifying sperm quality. The data obtained will lead to more accurate modeling of flagellum behavior during the motility period and provide a deeper understanding of basis of spermatozoa motility. The effect of water temperature on duration of embryo ontogenesis and hatching period; fertilization and development to the gastrula stage; hatching rates; and quality of larvae including larval size, development, and resistance to osmotic stress under controlled conditions for northern pike were investigated. Results indicated that the optimal temperature range for northern pike embryo development under controlled conditions is 6-10oC. Northern pike embryonic development hypothetically ceases at approximately 3.3 °C. In the certified methodology the basic aspects of controlled reproduction of northern pike was described and explained, including optimization of broodstock management. An objective of the certified methodology was to describe methods for producing high quality juveniles.
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