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Variabilita genu MYOD1 ve vztahu ke kvalitě masa u prasat
Španihelová, Věra
This bachelor's thesis is dedicated to exploring the correlation between the MYOD1 gene and meat quality traits in pigs. The MYOD1 gene, which is a member of the MYOD gene family, is involved in muscle development during myogenesis. Therefore, mutations in this gene may affect meat quality. For the study 46 DNA samples of Czech Large White pigs were used, and genotypes were obtained using the PCR-RFLP method, which involved digestion of the PCR product using the DdeI restriction endonuclease. The frequencies of the genotypes were calculated, with the AA genotype representing 19,6 %, AB representing 47,8 %, and BB representing 32,6 %. The most frequent allele was B, which was represented in 56,5 % of the samples.Statistical analysis was performed using the SAS program. The results indicated that only the myoglobin content in 100 g of muscle was affected by the MYOD1 gene with a P ≤ 0,05 level of significance. Specifically, a statistically significant difference was observed between the AA and AB genotypes, with individuals carrying the AA genotype exhibiting significantly lower myoglobin content.
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Analýza parametrů reprodukce ve velkochovu prasnic
KOŘÍNKOVÁ, Hana
In the thesis work have been compared with parameters of reproduction in sows of different genotype in the enterprise AGROPIG CZ s. r. o. in Velešín. There are in breeding were monitored achieved the performance parameters in sows of Czech white noble breed and crossbreed Czech white noble breed with landrace insemination hybrid boars. At the conclusion of the work was designed recommendations for the improvement of those parameters in practice. In the enterprise AGROPIG CZ s. r. o. have been identified following the results of the reproduction: there is in 2014 the breed BU, the average was stillborn 1,2 piglet of all births and losses in the postpartum period were 2,3 piglet. Crossbreed BU x L in this year have better values, was born to them in average of 1 stillborn piglet and losses of piglets in the postnatal period were at an average of 1.8 piglet. In 2015 the results of both groups are identical. The diameter of stillborn piglets was 0,9 pieces of all births and losses in the postpartum period were 1.3 piglet. In 2014 breed of BU showed on average a lower number of live born piglets (11,7 pcs) compared to crossbreed BU x L (12,9 pcs, i.e. by 1.2 piglet more). Weaned piglets was on average 0.6 more at crossbreed BU x L. In the year 2015 should crossbreed in average by 0.4-born piglets more than purebred sows of Czech white noble breed. Live born piglets was in average 0.5 more and all born about 0,4 more. From the obtained data prove that the percentage of farrowing and service in 2014 and 2015 in gilts crossbreed breed white noble x landrace is better than for pure-bred gilts breed white noble. There is in the category of sows should be in 2015, better results pure male. Furthermore, it was found that sows in a given enterprise overrun most often in the range of 18. 24. day of gestation. This corresponds to the length of one reproductive cycle and may be due to the poor quality of the insemination dose, or the wrong technique of insemination. Percent overrun was in the two study groups almost equal in both monitored years. In contrast, in the value of the abortion the two groups differed. To abortion occurred in 2014 more often in crossbreed, in 2015 in purebred sows. Statistical evaluation shows that the gilts and sows crossbreed BU x L is less overrun, have better results of pregnancy rate and even better killing it. Crossbreed BU x L also had better indicators related to births. Crossbreed had on average more of all births, live born and weaned piglets (at least by 0.4 piglet more).
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