National Repository of Grey Literature 5 records found  Search took 0.00 seconds. 
Interaction of bacterial lectins with human lung epithelium
Vyhnalová, Kateřina ; Hodek, Petr (advisor) ; Bořek Dohalská, Lucie (referee)
Cystic fibrosis (CF) is an autosomal recessive disease caused by mutations in the CFTR (cystic fibrosis transmembrane conductance regulator) gene encoding the same named chloride channel, which is present on the apical membrane of epithelial cells. As a consequence of these mutations, the transepithelial transport of ions and the regulation of other ion channels are impaired. Cystic fibrosis affects primarily respiratory system, where, as a result of the dysfunction of the CFTR protein, is an accumulation of excessively viscous mucus and altered glycosylation of saccharide structures on the surface of the lung epithelium. This pathological condition predisposes CF patients to bacterial lung infections, the leading cause of death in CF patients. The most common pathogen colonizing the respiratory system of CF patients is Pseudomonas aeruginosa (PA). For adhesion to host tissue, PA uses several virulence factors, including lectin PA-IIL, which exhibits an unusually high affinity for L-fucose. In this work the influence of selected monosaccharides and multivalent fucose-based inhibitors (glycoclusters) on PA-IIL binding to human lung epithelium was investigated. To assess the effect of these agens ex vivo, paraffin-embedded formalin-fixed human lung tissues (FFPE tissues) from a healthy individual and...
Interaction of bacterial lectins with human lung epithelium
Vyhnalová, Kateřina ; Hodek, Petr (advisor) ; Nosková, Libuše (referee)
Recessive autosomal disease cystic fibrosis (CF) is caused by a mutation in the CFTR gene ("regulator of cystic fibrosis transmembrane conductance"), which encodes the same named chloride channel. This mutation leads to incorrect ion transport, which causes the formation of an excessively viscous mucus on the surface of the airways and subsequently to the susceptibility to bacterial diseases. This disease mainly affects the respiratory system, where infections are associated with various causes of death in patients with CF. The most common pathogen causing infections is Pseudomonas aeruginosa (PA), which uses many virulence factors, such as pili or adhesins. Lectin PA-IIL, from the group of PA adhesins, is characterized by a high affinity for L-fucose, so it contributes to the adhesion of PA to the low sialylated epithelium of CF patients. In this work the interactions between PA-IIL and lung epithelium were investigated. The cell lines CuFi-1 (CF patient) and NuLi-1 (healthy individual), which were examined ex vivo, were used. A part of these cell lines were exposed to neuraminidase. The PA-IIL lectin was isolated from the E. coli cell line pET25_PAIIL and subsequently fluorescently labeled with DyLight 488. The activity of mentioned lectin was verified by red blood cell agglutination. The...
Translation of francophone comics
Vyhnalová, Kateřina ; Šotolová, Jovanka (advisor) ; Brunel, Aude (referee)
(in English): The present thesis titled Translation of francophone comics deals with the subject matter of comics translation, while applying both a theoretical and an empirical approach. Particular attention is paid especially to translations from French into Czech. Regarded the fact that Czech science has only scarcely studied the subject so far, the present thesis is conceived as a global introduction to the subject matter with particular focus on some of its partial aspects. The introduction to the theoretical part of the present study defines the main characteristic features of comics as a medium, as these features are closely linked to the particular challenges raised by its translations. The key chapters of the theoretical part go further to the core of the principal subject and focus on setting a general theoretical framework for translation analysis of specific problems linked with comics translations. The most common challenges faced by a comics translator are identified based on the defined characteristics of the Ninth Art and systemized in three main groups defined as technical, cultural and linguistic aspects of translation. While translating comics from French into Czech, the translator's work is also influenced by the radical differences in approaches to the Ninth Art in the Czech...
Conflict between biofuels support and food security
Vyhnalová, Kateřina ; Jílková, Jiřina (advisor) ; Pělucha, Martin (referee)
In recent years biofuels support is on the rise and is leading to rise in their productions. Biofuels are produced on land with agricultural products that is why there is rising land competition. Food prices are rising fast and food security is deteriorating. Hypothesis is that countries and international organizations are introducing measuress to guarantee food security. The aim of the thesis is to analyze interventions that respond to impacts of biofuel support in selected countries. The hypothesis is right. States are introducing measures to guarantee food security. Developed states give more financial donations to humanitarian institutions and support development of second generation biofuels that is not competitive with food production. Developing states implement for example increasing food export duties or prohibition of food exports, reducing food import duties and reducing tax levy on food. Some developing states have decided for food safeguarding.
Migration, crises and remittances
Vyhnalová, Kateřina ; Čermáková, Klára (advisor) ; Katreniaková, Dagmara (referee)
The aim of this thesis is found if there is a relationship between economic development of USA and the flow of remittance selected states. If remittance is influenced by economic development of USA, the flow of remittance will fall during financial. This hypothesis is supported by the fact that financial crisis influences more foreign workers than native workers. Rate of unemployment of immigrants is growing and because remittance probably will fall. The paper is underlaid by theories which explain origin and duration of imigration. Than I examine remittance and the impact of crisis on immigrants, the flow of immigrants and the flow of remittance. In the empirical part I choose states, on which I explore the relationship between the flow of remittance and economic development of USA.

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