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The role of HOG MAPK signaling pathway during osmotic stress in Saccharomyces cerevisiae
Vrbová, Michaela ; Pospíšek, Martin (advisor) ; Janderová, Blanka (referee)
THE ROLE OF HOG MAPK SIGNALING PATHWAY DURING OSMOTIC STRESS IN SACCHAROMYCES CEREVISIAE Budding yeast (Saccharomyces cerevisiae) cells utilize a conserved mitogen-activated protein kinase (MAPK) signaling cascade (the high- osmolarity glycerol or HOG pathway) during conditions of increased external osmolarity. It evokes cellular responses necessary to permit continued growth. Activation of HOG pathway with Hog1 MAP kinase results in production of glycerol to prevent dehydration and up regulation of other Hog1 dependent genes because of cell adaptation. We were trying to find difference in translation between wild-type cells and two mutants of hog1 gene before and after 0,4 M NaCl osmotic stress (2, 6, 30 min). We used deletion mutant hog1Δ and hog1-as mutant with point mutation which allows inhibition of Hog1 MAPK during presence of specific AS inhibitor. We tested AS inhibitor by plate test and have found optimal concentration of 5 μM for blocking Hog1 MAPK in hog1-as mutant. Translation profiling proves that osmotic stress decreases translation in general. Hog1Δ mutant and hog1-as AS inhibited mutant behave similarly and their translation recovers slower than the wild-type's. That confirms that HOG1 gene is important for cell recovery from the osmotic stress. Microarray analysis shows that Hog1 dependent...
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The role of HOG MAPK signaling pathway during osmotic stress in Saccharomyces cerevisiae
Vrbová, Michaela ; Pospíšek, Martin (advisor) ; Janderová, Blanka (referee)
THE ROLE OF HOG MAPK SIGNALING PATHWAY DURING OSMOTIC STRESS IN SACCHAROMYCES CEREVISIAE Budding yeast (Saccharomyces cerevisiae) cells utilize a conserved mitogen-activated protein kinase (MAPK) signaling cascade (the high- osmolarity glycerol or HOG pathway) during conditions of increased external osmolarity. It evokes cellular responses necessary to permit continued growth. Activation of HOG pathway with Hog1 MAP kinase results in production of glycerol to prevent dehydration and up regulation of other Hog1 dependent genes because of cell adaptation. We were trying to find difference in translation between wild-type cells and two mutants of hog1 gene before and after 0,4 M NaCl osmotic stress (2, 6, 30 min). We used deletion mutant hog1Δ and hog1-as mutant with point mutation which allows inhibition of Hog1 MAPK during presence of specific AS inhibitor. We tested AS inhibitor by plate test and have found optimal concentration of 5 μM for blocking Hog1 MAPK in hog1-as mutant. Translation profiling proves that osmotic stress decreases translation in general. Hog1Δ mutant and hog1-as AS inhibited mutant behave similarly and their translation recovers slower than the wild-type's. That confirms that HOG1 gene is important for cell recovery from the osmotic stress. Microarray analysis shows that Hog1 dependent...
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Bancassurance isues
Vrbová, Michaela ; Daňhel, Jaroslav (advisor) ; Ducháčková, Eva (referee)
The aim of my thesis Bancassurance isues is to provide a complex and comprehensive view of this area. Significant part of my thesis is contained by theoretical definitions and descriptions of bancassurance history and developement in the world and Czech Republic as well. In this part I specify particular cooperation models and levels between banks and assurance companies. I also present benefits and disadvantages of bancassurance for customers and both banks and assurance companies. The second part of my thesis is more practically focussed. It is devoted to actual financial and economical crises, its impact on bancassurance field and bancassurance holdings. There is also a analyses of czech bancassurance market as a conclusion .
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PPP projekty v České republice
Vrbová, Michaela ; Pavel, Jan (advisor)
Práce se zabývá problematikou Public Private Partnership (zkráceně PPP), tedy partnerstvím veřejného a soukromého sektoru. Teoretický koncept PPP projektů je popsán v úvodní části práce. Jsou zde rozebrány základní problémy, rizika a postupy při používání tohoto způsobu financování. Dále je rozebrán Public Sector Comparator a poslední část práce se zaměřuje na problematiku a zabezpečení projektů v rámci České republiky a aktuální vývoj vybraných projektů.
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