|
|
Comparison of differential leukocyte count set by the analyzer Beckman Coulter LH 750 and by microscope in the group of pediatric hemato- oncology patients
Šturmová, Michaela ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
In my thesis I focused on comparing a differential leukocyte count (DIF) determined by the analyzer and by microscope in the group of pediatric oncological and hemato-oncological patients. Thesis is divided into theoretical and practical parts. The theoretical part includes an explanation of the process of hematopoiesis, the development of blood cells and the characteristic of time series, a decription of hemato-oncological and oncological diseases which occur most frequently in the monitored group of pediatric patients, and their treatment. In a practical part is described a determination of the DIF by the analyzer Beckman Coulter LH 750 and by microscope Meopta. I measured a blood count with the differencial leukocyte count, I made a blood smear and I evaluated microscopically it, in the selected group of pediatric patients. The results were reported in the tables and statistically processed using paired t-test in GraphPad Prism and were created graphs. An aim of this thesis was to explore and evaluate further the differences between the two methods. In 19,32% of patients in the test group was not evaluated the DIF by the analyzer . They were patients with the fresh catch of acute leukemia, were they applied growth factors or were they the patients after chemotherapy. If in such cases analyzer...
|
|
|
Current possibilities of laboratory diagnostics of anemia
Šmídlová, Monika ; Vrbacký, Filip (advisor) ; Pešková, Eliška (referee)
Author: Monika Šmídlová Title: Current possibilities of laboratory diagnostics of anemia Type of assignment: Bachelor's thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Branch of study: Healthcare Laboratory Assistant (ZDLB) This bachelor's thesis discusses the general informations and summary of etiopathogenetical classification of anaemia and possibilities of laboratory diagnostics. Thesis is mainly focused on laboratory diagnosis of inherited types of anaemia. Anaemia or anaemia syndrome is a pathological condition characterized by low levels of haemoglobin and hematocrit under the physiological limit. Clinical manifestations are variously severe depending on development of disease and type of anaemia. Anaemia patient suffer from reduced oxygenation of tissues which determinates many symptoms of tissue hypoxia. Other symptoms are associated with hypovolemia and hyperkinetic circulation that can occur. In haemolytic anaemia, patients have symptoms of intravascular or extravascular haemolysis. Primary (hereditary) anaemia are congenital, the cause lies in genetic mutations that result a changes of phenotype properties of erythrocytes. The process of diagnosis determinating includes correct identification of family history, relevant medical examinations and laboratory...
|
|
|
Development of anticoagulation therapy and news about its monitoring
Klazarová, Alena ; Vrbacký, Filip (advisor) ; Sadílek, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Laboratory diagnostics in healthcare Author: Alena Klazarová Supervisor: RNDr. Filip Vrbacký, Ph.D. Title of bachelor thesis: Development of anticoagulation therapy and news about its monitoring Background: The aim of this work is to summarize the basic information about hemostasis and the components that participate in it. These facts are key to understanding the mechanism of action of anticoagulants in anticoagulant therapy. The work deals with an overview of the development of anticoagulant treatment from the beginning to the present day. It deals with the issue of anticoagulant treatment and the possibility of its monitoring by laboratory tests. Main findings: Thromboembolism suffered people more or less since time immemorial. The question of time was the development of anticoagulant therapy and an understanding of the mechanism of action of anticoagulant drugs, which was of great benefit in reducing the incidence and mortality of thromboembolism. Conclusions: The introduction of anticoagulant therapy in medicine has reduced the risk of thromboembolic complications. A great benefit was the discovery of the first anticoagulant drug, unfractionated heparin (UFH), followed by oral...
|
|
| |
|
|
Study of new prognostic markers in patients with chronic lymphocytic leukemia
Vrbacký, Filip
Title: Study of new prognostic markers in patients with chronic lymphocytic leukemia Chronic lymphocytic leukemia (CLL) is the most common leukemic disorder of adults in Western hemisphere. It is characterized by clonal proliferation and accumulation of morphologically mature lymphocytes in bone marrow, peripheral blood and lymphatic tissues. Clinical course of CLL is extremely heterogeneous with some patients living for decades without need of therapy while others succumbing to the disease within several years. Thus, there has been great interest in identifying prognostic markers that could be used to distinguish patients with an aggressive form of CLL, because they might benefit from early intervention. The process of angiogenesis has been shown to be crucial for growth and metastasizing ability of solid tumors. Angiogenesis in "liquid" tumors was supposed to be less important, nevertheless numerous recent studies have shown enhanced angiogenesis in many hematological malignancies including CLL. Elevated levels of circulating angiogenic cytokines and increased expression of genes encoding angiogenic factors have been reported in recent years in patients with chronic lymphocytic leukemia (CLL) but data regarding their prognostic and predictive significance are still limited. Therefore, in the...
|
|
| |
|
| |
|
|
Study of new prognostic markers in patients with chronic lymphocytic leukemia
Vrbacký, Filip
Title: Study of new prognostic markers in patients with chronic lymphocytic leukemia Chronic lymphocytic leukemia (CLL) is the most common leukemic disorder of adults in Western hemisphere. It is characterized by clonal proliferation and accumulation of morphologically mature lymphocytes in bone marrow, peripheral blood and lymphatic tissues. Clinical course of CLL is extremely heterogeneous with some patients living for decades without need of therapy while others succumbing to the disease within several years. Thus, there has been great interest in identifying prognostic markers that could be used to distinguish patients with an aggressive form of CLL, because they might benefit from early intervention. The process of angiogenesis has been shown to be crucial for growth and metastasizing ability of solid tumors. Angiogenesis in "liquid" tumors was supposed to be less important, nevertheless numerous recent studies have shown enhanced angiogenesis in many hematological malignancies including CLL. Elevated levels of circulating angiogenic cytokines and increased expression of genes encoding angiogenic factors have been reported in recent years in patients with chronic lymphocytic leukemia (CLL) but data regarding their prognostic and predictive significance are still limited. Therefore, in the...
|
|
|
Possibilities of platelet count evaluation by analyzer Sysmex XN in a group of patients with thrombocytopenia
Charvátová, Martina ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Martina Charvátová Supervisor: Mgr. Ilona Fátorová Title of diploma thesis: Possibilities of platelet count evaluation by analyzer Sysmex XN in a group of patients with thrombocytopenia This diploma thesis deals with the problems of platelet count evaluation in thrombocytopenic patients. There are several principles of determination of platelet count, which are currently used. The aim of this thesis is to assess and analyze three of them (impedance, optical and fluorescence method) in 400 patients. The theoretical part includes topics like function and development of platelets, principles of their count determination and pathology associated with low platelet count. In the experimental part the obtained data is graphically and statistically evaluated to analyse the agreement between three measurements. Methods: Analysis of complete blood counts was performed by the analyzer Sysmex XN-10. Platelet counts were obtained from PLT-I, PLT-O and PLT-F methods. Some blood samples required a microscopic control of platelet morfology. Results: The obtained data was statistically evaluated with help of Wilcoxon test and Bland- Altman plots. Between all three methods very good...
|
|
|
Study of new prognostic markers in patients with chronic lymphocytic leukemia
Vrbacký, Filip ; Červinka, Miroslav (advisor) ; Pospíšilová, Šárka (referee) ; Procházka, Vlastimil (referee)
3 Summary Title: Study of new prognostic markers in patients with chronic lymphocytic leukemia Chronic lymphocytic leukemia (CLL) is the most common leukemic disorder of adults in Western hemisphere. It is characterized by clonal proliferation and accumulation of morphologically mature lymphocytes in bone marrow, peripheral blood and lymphatic tissues. Clinical course of CLL is extremely heterogeneous with some patients living for decades without need of therapy while others succumbing to the disease within several years. Thus, there has been great interest in identifying prognostic markers that could be used to distinguish patients with an aggressive form of CLL, because they might benefit from early intervention. The process of angiogenesis has been shown to be crucial for growth and metastasizing ability of solid tumors. Angiogenesis in "liquid" tumors was supposed to be less important, nevertheless numerous recent studies have shown enhanced angiogenesis in many hematological malignancies including CLL. Elevated levels of circulating angiogenic cytokines and increased expression of genes encoding angiogenic factors have been reported in recent years in patients with chronic lymphocytic leukemia (CLL) but data regarding their prognostic and predictive significance are still limited. Therefore, in the...
|
guest ::
