National Repository of Grey Literature 14 records found  previous11 - 14  jump to record: Search took 0.01 seconds. 
Redox DNA Labeling - from Simple DNA Detection by Osmium Tetroxide Complexes Modification to Ratiometric Sequence Analysis
Havran, Luděk ; Balintová, Jana ; Vidláková, Pavlína ; Macíčková-Cahová, Hana ; Pivoňková, Hana ; Hocek, Michal ; Fojta, Miroslav
DNA is electroactive molecule producing analytically useful intrinsic voltammetric signals. For some applications is a useful use redox active tag to improve specificity of the analysis. One from approaches how to prepare DNA bearing redox label is its modification by complexes of osmium tetroxide with nitrogen ligands. This method find wide used in highly sensitive DNA detection and in development of electrochemical DNA hybridization sensors. Another possibility for preparation of redox labeled DNA is incorporation of redox tags modified deoxynucleotide triphosphates by DNA polymerases. In this contribution will be demonstrated application of various redox labels in electrochemical analysis of DNA.
Magnetic Beads-Based Electrochemical Techniques for DNA-Protein Interaction Monitoring
Fojta, Miroslav ; Pivoňková, Hana ; Němcová, Kateřina ; Horáková Brázdilová, Petra ; Havran, Luděk ; Orság, Petr ; Vidláková, Pavlína ; Macíčková-Cahová, Hana ; Balintová, Jana ; Hocek, Michal
Electrochemical techniques, in connection with separation of nucleoprotein complexes at magnetic beads, are suitable for the monitoring of DNA-protein interactions. For the detection of complexes captured at the beads it is possible to utilize intrinsic electrochemical activity of the protein, intrinsic structure-selective signals of the DNA, or indicator DNA substrates tail-labeled with electroactive moieties.
Using Anthraquinone Labeled Nucleotide Triphosphates for Electrochemical Analysis of DNA
Vidláková, Pavlína ; Balintová, Jana ; Horáková Brázdilová, Petra ; Havran, Luděk ; Orság, Petr ; Fojta, Miroslav ; Hocek, Michal
Base-modified anthraquinone-nucleotide triphosphates were used for DNA labeling. Anthraquinone modified nucleotides were incorporated into DNA by primer extension with KOD polymerase. In cyclic and square wave voltammetry at HMDE or PGE antraquinone gave a well developed characteristic signal suitable for differentiation of the labeled DNA from unlabeled one.
Electrochemical analysis of DNA using switchable redox moieties
Fojta, Miroslav ; Daňhel, Aleš ; Horáková Brázdilová, Petra ; Plucnara, Medard ; Pivoňková, Hana ; Havran, Luděk ; Vidláková, Pavlína ; Raindlová, Veronika ; Balintová, Jana ; Macíčková-Cahová, Hana ; Hocek, Michal
Labelling of DNA with electrochemically active moieties proved to be a convenient way to the development of electrochemical techniques for the sequence-specific DNA sensing. Through combinations of various labels differing in redox potentials, independent redox coding of different DNA sequences or individual nucleobases can be attained. Applications possibilities of electrochemistry in analysis of modified DNAs are further extended by facile monitoring of chemical conversion of reactive groups on DNA during post-labelling with ultimate redox labels. In addition, controlled in situ electrochemical conversions of specific intrinsic and extrinsic DNA components can be utilized to switch their electrochemical signals and improve signal resolution.

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1 Vidláková, Petra
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