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Simulations of light scattering from living cells
Vengh, Martin ; Richter, Ivan (referee) ; Petráček, Jiří (advisor)
Diplomová práca sa zaoberá rozptylom elektromagnetického žiarenia na biologickej bunke použitím metódy konečných diferencií v časovej oblasti (FDTD), Bornovej aproximácie a Rytovovej aproximácie. Metóda FDTD dáva presné výsledky v širokej škále problémov. Je spravené porovnanie Bornovej aproximácie a Rytovovej aproximácie prostredníctvom FDTD metódy. Ďaľšia časť práce zahrnuje krátky popis koherenciou riadeného holografického mikroskopu CCHM. Záverečná časť sa venuje zobrazeniu rozptýleného poľa získaného z jednotlivých simulácií pomocou simulácie objektového ramena mikroskopu CCHM.
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Observation of Influence of Thin Layers on Cell Behaviour by Multimodal Holographic Microscope
Vengh, Martin ; Křížová, Aneta (referee) ; Štrbková, Lenka (advisor)
Surface treatment of materials for the cell-surface interaction and imitating intrinsic environment of cells is still subject of matter for their potentional usage in the bioengineering. One of the options to modify the surface of material is exhibiting them to effects of plasma of electrical discharge in steam of cyclopropylamine. In this type of plasma, the low pressure plasma polymerization takes place, which creates thin amine-rich layer. There is an assumption that this type of layer is appropriate for cell adhesion and proliferation. As a superior technique for a label-free monitoring of the cell-surface interaction, multimodal holographic microscope (MHM) Q-Phase was exploited to determine the biocompatibility of materials. MHM enables observation in quantitative phase imaging, where the phase is directly proportional to the cell dry mass. This gives opportunity to define various features able to determine the biocompatibility of materials. According to the results amine-rich films enhanced the conditions for the cell adhesion and proliferation.
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Simulations of light scattering from living cells
Vengh, Martin ; Richter, Ivan (referee) ; Petráček, Jiří (advisor)
Diplomová práca sa zaoberá rozptylom elektromagnetického žiarenia na biologickej bunke použitím metódy konečných diferencií v časovej oblasti (FDTD), Bornovej aproximácie a Rytovovej aproximácie. Metóda FDTD dáva presné výsledky v širokej škále problémov. Je spravené porovnanie Bornovej aproximácie a Rytovovej aproximácie prostredníctvom FDTD metódy. Ďaľšia časť práce zahrnuje krátky popis koherenciou riadeného holografického mikroskopu CCHM. Záverečná časť sa venuje zobrazeniu rozptýleného poľa získaného z jednotlivých simulácií pomocou simulácie objektového ramena mikroskopu CCHM.
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Observation of Influence of Thin Layers on Cell Behaviour by Multimodal Holographic Microscope
Vengh, Martin ; Křížová, Aneta (referee) ; Štrbková, Lenka (advisor)
Surface treatment of materials for the cell-surface interaction and imitating intrinsic environment of cells is still subject of matter for their potentional usage in the bioengineering. One of the options to modify the surface of material is exhibiting them to effects of plasma of electrical discharge in steam of cyclopropylamine. In this type of plasma, the low pressure plasma polymerization takes place, which creates thin amine-rich layer. There is an assumption that this type of layer is appropriate for cell adhesion and proliferation. As a superior technique for a label-free monitoring of the cell-surface interaction, multimodal holographic microscope (MHM) Q-Phase was exploited to determine the biocompatibility of materials. MHM enables observation in quantitative phase imaging, where the phase is directly proportional to the cell dry mass. This gives opportunity to define various features able to determine the biocompatibility of materials. According to the results amine-rich films enhanced the conditions for the cell adhesion and proliferation.
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