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NAD+-dependent histone deacetylase SIRT1 in the process of oogenesis, fertilization and early embryonic development
Valentová, Iveta ; Nevoral, Jan (advisor) ; Drutovič, David (referee)
SIRT1 is a histone deacetylase from the sirtuin family that affects epigenetic and non- epigenetic targets. We can assume that the known SIRT1 substrates are involved in the regulation of gametogenesis and early embryonic development. Our hypotheses say SIRT1 is present in oocytes and early embryos and it plays a physiological role in oocyte maturation, fertilization and early embryonic development. A mouse model of a conditional knock-out line producing Sirt1-deficient oocytes was developed to verify our hypotheses. Oocytes and embryos were analyzed for SIRT1, its selected substrates and other markers by immunocytochemistry. We found out that the presence of SIRT1 contributes to oocyte quality through modulation of the chromatin histone code and stabilization of the spindle. Furthermore, the purely maternal origin of SIRT1 presents in both zygote pronuclei. Last but not least we discovered a significant effect of SIRT1 on early embryonic development, probably mainly due to its role in the activation of the embryonic genome. The results confirm our hypothesis that SIRT1 is present in oocytes and embryos mainly around chromatin. The results show that SIRT1 is a maternal factor determining oocyte quality and it is necessary for the embryonic genome activation.
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PPPprojects in the Czech Republic
Valentová, Iveta ; Ochrana, František (advisor) ; Witz, Petr (referee)
The diploma work addresses the cooperation between public and private sector called PPP projects. This tool of financing comes out of generally known public contracts, however it puts more competence to the private sector. The aim of this work is to describe and explain how PPP projects works, what advantages or disadvantages are connected with them. This Work works with a hypothesis that PPP projects are suitable for financing and run of senior homes. The number of senior homes is currently low and therefore they do not cover the actual demand. Theoretical outcomes come from the theory of New Public Management, public interest, public services and public politics. It is based especially on semistructured interviews with experts that are involved with current topic, secondary data analysis and study ofdocuments. In the end of the work is explained how PPP projects can be useful for operating senior homes.
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PPPprojects in the Czech Republic
Valentová, Iveta ; Ochrana, František (advisor) ; Witz, Petr (referee)
The diploma work addresses the cooperation between public and private sector called PPP projects. This tool of financing comes out of generally known public contracts, however it puts more competence to the private sector. The aim of this work is to describe and explain how PPP projects works, what advantages or disadvantages are connected with them. This Work works with a hypothesis that PPP projects are suitable for financing and run of senior homes. The number of senior homes is currently low and therefore they do not cover the actual demand. Theoretical outcomes come from the theory of New Public Management, public interest, public services and public politics. It is based especially on semistructured interviews with experts that are involved with current topic, secondary data analysis and study ofdocuments. In the end of the work is explained how PPP projects can be useful for operating senior homes.
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