National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Imunomagnetic separation of lactic acid bacteria using magnetic microparticles functionalised by antibodies
Vaňásek, Jakub ; Španová, Alena (referee) ; Trachtová, Štěpánka (advisor)
Immunomagnetic separation is based on binding of antibody with antigen, where antibody is bound to magnetic particle. In this thesis there were used particles of magnetic pearl cellulose with antiLactobacillus and antiBifidobacterium antibodies. Immunomagnetic separation method was optimalized and verified for its efficiency and specifity with bacterial and yeast cells. This cells were identified by polymerase chain reaction. Efficiency of immunomagnetic separation was verified on probiotic meat product, where Lactobacillus cells were isolated. With DNA from isolated Lactobacillus cells the high resolution melting was performed. The results show presence of several bacterial strains of Lactobacillus species.
The application of microorganisms to the degradation of lipids
Vaňásek, Jakub ; Voběrková, Stanislava (referee) ; Omelková, Jiřina (advisor)
This bachelor thesis is focused on the study of commercial products for degradation of lipids in waste water. This commercial products contain microorganisms with lipolytic activity. Theoretical part of this thesis describes lipids, lipases, mikroorganisms which produce lipolytic enzymes and basical principles of cleaning of waste water. In this part analytical methods of lipids isolation are presented too. The practical part of this thesis is concerned with the study of three commercial products. These products were tested for the content of microflora. Subsequently the restriction analysis was performed. Using this method the microorganisms were identified as Bacillus sp. Furthermore the growth curves were determined. This curves show growth of biomass. Then the pH during cultivation and lipolytic activity of microorganisms were determined. Lipolytic activity was determined by spectrofotometric method with using of p-nitrophenyllaurate.

See also: similar author names
1 VAŇÁSEK, Jiří
4 Vaňásek, Jaroslav
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