National Repository of Grey Literature 5 records found  Search took 0.00 seconds. 
The detection of food with gene manipulated organisms in chicken
VRABCOVÁ, Petra
The aim of this study is to confirm or to refutate possible transfer of transgene from diet containing genetically modified organism in blood of broilers. Broilers were fed with diets containing Roundup Ready soybean and Bt maize (MON810). DNA was isolated from the whole blood of these broilers. PCR using primers for growth hormon was performed as control of isolation. Then samples were submitted to PCR for detection of fragments of lectin genes from soya and HMG gene from maize. Fragments of soya DNA and maize DNA in the blood were detected. Subsequently, the detection of specific transgene of RoundupReady soya and Bt maize (MON810) in blood samples was positive too.
New cryoprotectives in preservation of potentially probiotic bacteria of Bifidobacterium genus using freeze-drying procedure
Vrabcová, Petra ; Killer, Jiří (advisor) ; Jakub, Jakub (referee)
The probiotic bacteria of the genus Bifidobacterium are especially used in the dairy and pharmaceutical industry. They are present in the form of viable cells in pro(syn)biotic fermented milk, dried and freeze-dried products for its positive effects on the health. Lyophilization represents one of the widely used methods for long-term preservation of bifidobacteria. The lyophilization process is challenging, but very needed, and for this reason it is still necessary to explore new cryo(lyo)protective substances, which protect the microbial cultures more effectively before the negative effects of cryopreservation and lyophilization. The aim of the thesis was to test the various mixtures of substances containing specific oligosaccharides, which have prebiotic properties, from the point of view of their potential cryo(lyo)protective effect of lyophilization process. For the purposes of the study, twelve bifidobacterial strains of human and animal origin were selected. As a potential cryo(lyo)protectives were used solutions on the basis of 5x concentrated TPY medium with addition of FOS, 10% fructooligosaccharides (FOS) and the solution on the basis of porcine gastric mucin. Number of viable cells was determined in lyophilisates enriched by the concrete solutions in the time intervals (30 days, 3 months and 6 months) after lyophilization and compared with the control lyophilized samples (fresh cultures without the additive solutions). The potential cryo(lyo)protective effectiveness of 5x concentrated TPY medium solution has been demonstrated only in B. boum, B. breve, B. longum subsp. infantis a Bombiscardovia coagulans, where decreased the number of viable cells about four orders of magnitude. The potential cryo(lyo)protective effectiveness in 10% FOS solution was found only in B. thermacidophilum subsp. porcinum and Bombiscardovia coagulans, where was found half the number of viable cells. The highest potential cryo(lyo)protective effectiveness was observed for a solution on the basis of porcine gastric mucin. The highest number of viable cells was demonstrated in B. animalis subsp. lactis, B. boum, B. longum subsp. infantis, B. longum subsp. longum, B. pseudolongum subsp. globosum and B. thermacidophilum subsp. porcinum lyophilisates using this potential cryo(lyo)protective solution.

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5 Vrabcová, Petra
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