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Methods for study of plant phosphoproteins
Válková, Martina ; Lochman,, Jan (referee) ; Fohlerová, Radka (advisor)
Reversibly phosphorylated proteins play a role in many fundamental cellular processes. An example is the cytokinin signal transduction pathway in plants that is mediated by the so-called two-component system involving histidine and aspartate phosphorylation. Due to the very low stability of phosphorylation of these amino acids, is difficult to find a suitable method for phosphorylation studies. The theoretical part of this bachelor thesis describes not only the current experimental approaches for analysis of protein phosphorylation in two-component systems but also is trying to propose new approaches for this analysis. There are described methods based on labeling of proteins using [?-32P] ATP and on the detection of released phosphate employing the colorimetric method or ICP-MS. Another possibility is sensitive identification of phoshorylated and non-phosphorylated forms of protein fused with GFP using CE-LIF technique. The practical part of the thesis deals with optimization of the selected colorimetric methods and also is focused on the preparation of the AHP5 protein fused with GFP for CE-LIF analysis.
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Attacks based on hardware bugs
Válková, Martina ; Holub, Štěpán (advisor) ; Příhoda, Pavel (referee)
Title: Attacks based on hardware bugs Author: Martina Válková Department: Department of Algebra Supervisor: Mgr. Štěpán Holub, Ph.D. Supervisor's e-mail address: Stepan.Holub@mff.cuni.cz Abstract: The study concerns hardware bugs producing computational errors and cryptanalytic attacks which utilize them. Particularly, the research is focused on attacks presented in the article by Biham E., Carmeli Y., Shamir A.: Bug Attacks [1] and their practical application in the case of schemes RSA and Pohlig-Hellman and various computational circumstances, which points out bigger vulnerability of schemes in the case of using the Right- to-Left modular exponentiation algorithm. The attacks have been tested against the software simulation of a faulty processor, which confirmed that they pose a real security threat in point of that situation. The mathematical part of this work concerns the problem of the finding any roots in Zp. Keywords: hardware bug, attack, RSA, roots modulo p
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Methods for study of plant phosphoproteins
Válková, Martina ; Lochman,, Jan (referee) ; Fohlerová, Radka (advisor)
Reversibly phosphorylated proteins play a role in many fundamental cellular processes. An example is the cytokinin signal transduction pathway in plants that is mediated by the so-called two-component system involving histidine and aspartate phosphorylation. Due to the very low stability of phosphorylation of these amino acids, is difficult to find a suitable method for phosphorylation studies. The theoretical part of this bachelor thesis describes not only the current experimental approaches for analysis of protein phosphorylation in two-component systems but also is trying to propose new approaches for this analysis. There are described methods based on labeling of proteins using [?-32P] ATP and on the detection of released phosphate employing the colorimetric method or ICP-MS. Another possibility is sensitive identification of phoshorylated and non-phosphorylated forms of protein fused with GFP using CE-LIF technique. The practical part of the thesis deals with optimization of the selected colorimetric methods and also is focused on the preparation of the AHP5 protein fused with GFP for CE-LIF analysis.
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