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Use of Chiral Separations for the Determination of Enzyme Enantioselectivity
Břicháč, Jiří ; Zima, Jiří (advisor) ; Tesařová, Eva (referee) ; Flieger, Miroslav (referee)
Conclusion Enantioselective enzymes pray important rore in metaboric pathways of chirar compounds. Enantioselective metabolism of lipid peroxidation product HNE was studied using measurement of enzyme kinetics. Difference in ability to detoxiý individua| HNE enantiomers by rat brain mitochondriar ALDHs was found. Based on kinetic parameters of purified recombinant enz),Ťnes' it was concluded, that rat ALDH5A enantioselective|y oxidized (R)-HNE' whereas rat ALDH2 was not enantioserective. Moreover, direct and indirect RP-HPLC methods for the separation of HNEA enantiomers were deveroped and validated. The indirect method involved pre-corumn derivatization with a chiral amino agent ANPAD' and subsequent separation ofdiastereomers on a Spherisorb oDS2 column. The direct separation of HNEA enantiomers was performed using the porysacharide-based CSP, Chiralpak AD-RH. Enantioserective enzymes are useful biocatalyzators a'owing cheap preparation of optically pure chemicals. screening of epoxide hydrolases produced by various microorganisms was performed and enantioselective enzymes were found using chiral GC on cyclodextrin-based CSps. In summary, this thesis describes development and optimization of the analytical methods for determination of various chiral compounds implicated in enzyme enantioselectiviý. All...
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Evaluation of the Financial Situation of the Company and Proposals to its Improvement
Čuperová Šebková, Lenka ; Tesařová, Eva (referee) ; Pernica, Martin (advisor)
This bachelor's thesis deals with the evaluation of the financial situation of the company in between years 2012 and 2015, which for 2015 are known to preliminary results. In the first part is described theoretical knowledge about the various ways a comprehensive evaluation of the financial situation of the company. And the second part is a description of the company and evaluated factors affecting in market position. The third part contains calculations of individual indicators, interpretation of results and SWOT analysis. The conclusion is contained proposals to improve the financial management of the company.
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Charakteristika a srovnání přístupu francouzské a české generace Y k pracovnímu uplatnění.
Tesařová, Eva
The diploma thesis deals with the generation Y and its approach to employment. The theoretical part is devoted to generational theories and characteristics of existing generations and detailed focus on generation Y. Practical part focus on surveys realized on the topic generation Y in the labour market. There is also own survey in the practical part. The goal of the survey is the comparison of generation Y in Czech Republic and French and its approach to labour market. Based on the results of the researches, proposals are recommended for employers to introduce new instruments for adaptation generation Y in the workplace. Consequently there is assessment of costs for these proposals.
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In-company accountancy as a basis of managerial determination
TESAŘOVÁ, Eva
This thesis is focused on the accounting system and its influence on management. The aim was to apply the method of cost allocation and use of the outputs of internal accounting of the company Omega a.s. Further calculations were made of selected financial indicators on which the assessment was carried out in-house staff. Obtained accounting data are processed by the form of tables and accounts.
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