National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Interactions of polyomavirus structures in the endoplasmic reticulum and on the path to the nucleus
Svobodová, Terezie ; Huerfano Meneses, Sandra (advisor) ; Weber, Jan (referee)
Mouse polyomavirus is a member and model virus of Polyomaviridae family. In order to infect cells and produce viral progeny, the viral chromosome must be transported to the nucleus. Several studies suggest that virions are transporeted to the endoplasmic reticulum, from which they are transferred to the cytosol with assistace of host proteins. Two of these proteins are the chaperon, BiP (binding immunoglobulin protein) and the cochaperone, DNAJ B14. Polyomaviruses probably enter the nucleus through nuclear pores with the assistence of importins. These processes were mainly studied with SV40. In this work, we show that MPyV infection induces a change in distribution of the DNAJ B14 protein, which became clustered into foci, where it co-localizes with the viral capsid protein, VP1. The occurrence of foci varies during infection. With use of proximity ligation assay, we have shown that during an early fase of MPyV infection, DNAJ B14 and BiP get in the close proximity with VP1. It is suggested that negatively charged amino acids at the N-terminus of the minor capsid protein, VP2, are required for targeting virions to translocon and proteins associated with ERAD. We created MPyV with VP2 mutated in these amino acids. The negatively charged amino acid at position 17 is not necessary for successful...
Interactions of polyomavirus structures in the endoplasmic reticulum and on the path to the nucleus
Svobodová, Terezie ; Huerfano Meneses, Sandra (advisor) ; Weber, Jan (referee)
Mouse polyomavirus is a member and model virus of Polyomaviridae family. In order to infect cells and produce viral progeny, the viral chromosome must be transported to the nucleus. Several studies suggest that virions are transporeted to the endoplasmic reticulum, from which they are transferred to the cytosol with assistace of host proteins. Two of these proteins are the chaperon, BiP (binding immunoglobulin protein) and the cochaperone, DNAJ B14. Polyomaviruses probably enter the nucleus through nuclear pores with the assistence of importins. These processes were mainly studied with SV40. In this work, we show that MPyV infection induces a change in distribution of the DNAJ B14 protein, which became clustered into foci, where it co-localizes with the viral capsid protein, VP1. The occurrence of foci varies during infection. With use of proximity ligation assay, we have shown that during an early fase of MPyV infection, DNAJ B14 and BiP get in the close proximity with VP1. It is suggested that negatively charged amino acids at the N-terminus of the minor capsid protein, VP2, are required for targeting virions to translocon and proteins associated with ERAD. We created MPyV with VP2 mutated in these amino acids. The negatively charged amino acid at position 17 is not necessary for successful...
Endoplasmic reticulum-associated degradation and its role in virus infection
Svobodová, Terezie ; Forstová, Jitka (advisor) ; Mašek, Tomáš (referee)
The endoplasmic reticulum-associated degradation pathway, ERAD, is an important mechanism for maintaining cellular homeostasis. The function of ERAD is degradation of accumulated unfolded proteins in the endoplasmic reticulum. ERAD is carefully regulated by pathway called "Unfold protein response" and by "ERAD tuning" mechanism. Some viruses have adopted the ways how to exploit this system or its factors for their own benefit. These utilizations include targeting of specific host proteins for degradation, transfer of viral products or virions from the endoplasmic reticulum into the cytoplasm, or the use of a membrane platform arising from the cooperation with "ERAD tuning" for viral replication. Role of ERAD in viral infection can manifest itself in different ways, it can contribute to degradation not only of host proteins but also of viral products. In this work I summarize mechanisms of ERAD pathway and their regulatory pathways. Meanwhile, in the specific examples, I present roles of ERAD pathway and associated systems in viral infections.

See also: similar author names
50 SVOBODOVÁ, Tereza
1 Svobodová, Tamara
50 Svobodová, Tereza
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