National Repository of Grey Literature 235 records found  previous11 - 20nextend  jump to record: Search took 0.01 seconds. 
The impact of selected sesquiterpenes on the activity of antioxidant enzymes in the differentiated Caco-2 cell line
Verešová, Erika ; Svobodová, Hana (advisor) ; Szotáková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Erika Verešová Supervisor: PharmDr. Hana Bártíková, Ph.D. Title of thesis: The impact of selected sesquiterpenes on the activity of antioxidant enzymes in the differentiated Caco-2 cell line The cases of cancer diagnosis rapidly continue to grow across the population. It is a modern trend to buy more and more often a variety of herbal food supplements in order to strengthen or restore human health. Many scientists have been researching Myrica rubra plant for its potential therapeutic effects. In our research we used sesquiterpenes isolated from this plant, in order to determine their effects on the activity levels of antioxidant enzymes in a differentiated Caco-2 cell line derived from intestinal epithelium tumor that have maintained characteristics of healthy cells. Caco-2 cells are being used as a basis for testing of substances and their effects on non-cancerous cells. The cells were incubated for 24 and 48 hours with a concentration 25 µg/ml of tested substances. Essential oil from Myrica rubra and selected individual sesquiterpenes (α- humulene, β-caryophyllene and trans-nerolidol) were chosen to be tested. Of the antioxidant enzymes, we determined the enzymes glutathione-S-transferase,...
Surface expression of Tim-3 inhibitory molecule on antigen-specific CD8+ T cells expanded in vitro using dendritic cells for cell-based cancer immunotherapy
Svobodová, Hana ; Smrž, Daniel (advisor) ; Funda, David (referee)
Cancer is the second most common cause of death in the world, and the number of people with the disease increases each year. The therapy of the disease currently stands on four pillars; surgery, chemotherapy, radiotherapy, and immunotherapy. Through the past few years, immunotherapy has become the fastest developing treatment modality. However, despite its unprecedented efficacy in some patients, the majority of patients still does not respond to the therapy. Therefore, there is a need to investigate the mechanisms that make immunotherapy inefficient. Cell-based cancer immunotherapy is the treatment modality which uses live ex vivo-produced tumor-targeting immune cells to treat cancer. One of the mechanisms that may compromise its therapeutic efficacy is the expression of inhibitory molecules on the surface of the produced immune cells. Tim-3 is the inhibitory molecule which attracts attention in recent years. Tim-3 expression in the tumor cells and the tumor-infiltrating immune cells is often associated with worse prognosis and more aggressive forms of the disease. However, its role in the in vitro or ex vivo-produced immune cells is difficult to predict. In this work, an in vitro study model which is based on in vitro-produced antigen-specific CD8+ T cells with high expression of Tim-3 has been...
Modification of selected biotransformation enzymes during ageing (in rat)
Vyskočilová, Erika ; Boušová, Iva (advisor) ; Svobodová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Erika Vyskočilová Supervisor: PharmDr. Iva Boušová, Ph.D. Title of diploma thesis: MODIFICATION OF SELECTED BIOTRANSFORMATION ENZYMES DURING AGEING (IN RAT) Ageing is an inevitable biological process characterized by progressive functional decline of all organ systems with simultaneous increase in oxidative damage as a result of reactive oxygen species (ROS) accumulation. Increased activity of antioxidant enzymes, such as peroxidase and catalase, is related to increased production of ROS during ageing. Activity of biotransformation enzymes such as cytochrome P450 (CYP) and glutathion-S-transferase (GST) changes during ageing too. In general, activity of enzymes is lower in very young and very old individuals than in the period of adulthood. The aim of the present work was to study age-related changes in activities and expression of selected xenobiotic-metabolizing enzymes (GST, CYP 1A, CYP 2B, CYP 3A4) and antioxidant enzymes (catalase, peroxidase, superoxiddismutase) in rat liver. Male Wistar rats were sacrificed by decapitation under ether anesthesia at the age of 6 weeks and 21 months (6 individuals in each group). Subsequently, liver specimens were processed to obtain the subcellular...
The view of non-medical healthcare workers on the Czech nursing association's activity
Češek, Michal ; Svobodová, Hana (advisor) ; Šochmanová, Martina (referee)
In this thesis I deal with the theme of The Czech nursing association's activity that could relate to a dissatisfaction of non-medical healthcare workers with this association and its drop in a member base. The main aim of this thesis is to find out how non-medical healthcare workers perceive the activity of this association. While working on this thesis I draw inspiration from literature focused on the history of nursing organizations, a steady development of The Czech nursing association and its activities. To solve the aim of this research I ran a quantitative research method of electronic and paper questionnaires that were sent out to several of non-medical healthcare workers. Results of this thesis suggest that the negative attitude of non-medical healthcare workers to The Czech nursing association's activities indeed relate to its activity and presentation. Outcomes of this thesis could serve as a self-reflection and a potential way on how to deal with a cause of a fluctuation of members of this association and regain the confidence of non-medical healthcare workers.
Influence of TNF-α on hENaC subunits expression
Martan, David ; Svobodová, Hana (advisor) ; Matoušková, Petra (referee)
University of Vienna Faculty of Life Sciences Department of Pharmacology and Toxicology Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: David Martan Supervisors: Prof. Dr. Rosa Lemmens-Gruber, PharmDr. Hana Bártíková, Ph.D. Title of the diploma thesis: Influence of TNF-α on hENaC subunits expression The human amiloride-sensitive epithelial sodium channel (hENaC) is a type of ion channel which has the ability to control salt and water homeostasis. Therefore it is one of the main driving forces for the reabsorption of water through the alveolar epithelium. A dysfunction of this channel, respectively of this control mechanism, leads to a very severe disease - pulmonary edema and several other pathological conditions. Previous studies tested a drug called AP301, recently named solnatide. AP301 is a cyclic protein comprising the human tumour necrosis factor lectin-like domain sequence. This drug was recently developed as a potential treatment of pulmonary edema. The principle is that it activates hENaC by increasing the open probability. It was also shown that AP301 transiently increases the expression of hENaC subunits in mammalian cells. In this study we used the Western blot method to test the influence of tumour necrosis factor α...
The impact of selected isoflavonoids on the effectiveness of anticancer therapy in MDA-MB-231 cell line
Lokvencová, Kateřina ; Svobodová, Hana (advisor) ; Matoušková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Kateřina Lokvencová Title, Name, Surname of tutor: PharmDr. Hana Bártíková, Ph.D. Title of a diploma work: The impact of selected isoflavonoids on the effectiveness of anticancer therapy in MDA-MB-231 cell line. Breast cancer is the most common cancer disease in women. There is an extensive amount of therapeutic regimens for the treatment of this disease, but it is often limited by lack of efficiency or serious side effects. Isoflavonoids genistein (GEN), daidzein (DAID) and formononetin (FORM) belong among the natural substances with bioprotective effects on the human body. Some epidemiological studies have confirmed the protective effect of isoflavonoids against certain types of cancer, including breast cancer. The aim of this work was to study the effect of isoflavonoids on cancer cells and assess their ability to support the effect of cytostatics, namely anthracycline antineoplastic agents doxorubicin (Dox). For the experiments was chosen the breast cancer cell line MDA-MB-231. To study the effect of isoflavonoids on tumor cells we chose concentrations of 1 and 10 µM which is according to the bioavailability of isoflavonoids. We have shown that isoflavonoids...

National Repository of Grey Literature : 235 records found   previous11 - 20nextend  jump to record:
See also: similar author names
41 SVOBODOVÁ, Hana
20 SVOBODOVÁ, Helena
2 Svobodová, Hana,
2 Svobodová, Hedvika
20 Svobodová, Helena
3 Svobodová, Hermína
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