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Optimalization of HPLC Separations of Basic Molecules of Betablockers - application of New Types of Sationary Phases
Svoboda, Antonín ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Antonín Svoboda Consultant: PharmDr. Lucie Nováková, Ph.D. Title of thesis: Optimalization of HPLC separations of basic molecules of betablockers - application of new types of sationary phases Beta blockers are pharmaceutically active substances, which affect on betaadrenergic system of human bodies. These drugs are used for various indications, particularly for the management of hypertension and cardiac arrythmias. The substances are chemically mostly basic non-polar compounds. In this thesis HPLC separation of seven betablockers on different stationary phases was tested. The optimal conditions for separation were found by changing composition, pH and elution mode of mobile phases. In some cases temperature was also optimized as a variable. The different conditions were compared in terms of retention, resolution and symmetry of peaks. The stationary phases were compared by System suitability test under the optimal found conditions. The results were acceptable on the Extra Densely Bonded and Double Endcapped C18 stationary phase Zorbax Eclipse-XDB. Zorbax Extend C18 column incorporating a patented bidentate silane with double- endcapping protection enabled measurements...
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Optimalization of HPLC Separations of Basic Molecules of Betablockers - application of New Types of Sationary Phases
Svoboda, Antonín ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Antonín Svoboda Consultant: PharmDr. Lucie Nováková, Ph.D. Title of thesis: Optimalization of HPLC separations of basic molecules of betablockers - application of new types of sationary phases Beta blockers are pharmaceutically active substances, which affect on betaadrenergic system of human bodies. These drugs are used for various indications, particularly for the management of hypertension and cardiac arrythmias. The substances are chemically mostly basic non-polar compounds. In this thesis HPLC separation of seven betablockers on different stationary phases was tested. The optimal conditions for separation were found by changing composition, pH and elution mode of mobile phases. In some cases temperature was also optimized as a variable. The different conditions were compared in terms of retention, resolution and symmetry of peaks. The stationary phases were compared by System suitability test under the optimal found conditions. The results were acceptable on the Extra Densely Bonded and Double Endcapped C18 stationary phase Zorbax Eclipse-XDB. Zorbax Extend C18 column incorporating a patented bidentate silane with double- endcapping protection enabled measurements...
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