|
|
Identification of new biomarkers and target molecules for the comprehensive treatment (drug-target) via proteomic analysis of body fluids and tissues experimental study
Fremuth, Jiří ; Kobr, Jiří (advisor) ; Nosáľ, Slavomír (referee) ; Hoza, Jozef (referee) ; Slavík, Zdeněk (referee)
The main objective of our prospective experimental study was to develop a clinically relevant model of mechanical ventilation of healthy and damaged lungs ventilated for 12 hours. A widely recommended Vt=6ml/kg was used in comparison with commonly used Vt=10ml/kg. The study included a total of 52 piglets of domestic pig (Sus scrofa f. domestica) age 5-9 weeks. Piglets were divided into 3 groups: group B - Vt = 6ml/kg (11 piglets, 8 died), group C - Vt = 10ml/kg (21 piglets, 5 died) and group D - Vt= 50 ml + instillation of 50 ml saline into the trachea (6 piglets, 1 died). In the presented study we focused on the influence of mechanical ventilation on several organ systems as well as induction of systemic inflammatory reaction. By using higher tidal volume, we aimed to induce possible biotraumatic changes. The ventilator settings were deliberately not changed during the study, except the change of Fi02 values based on the Sp02 value. The first goal was to assess the influence of two different strategies of mechanical ventilation and induced lung injury on respiratory functions of the lungs and pulmonary mechanics. Piglets ventilated with Vt = 6ml/kg maintain the physiological oxemia, but the level of capnia increased over time with an adequate effect on acid-base balance. The highest mortality in...
|
|
|
Identification of new biomarkers and target molecules for the comprehensive treatment (drug-target) via proteomic analysis of body fluids and tissues experimental study
Fremuth, Jiří ; Kobr, Jiří (advisor) ; Nosáľ, Slavomír (referee) ; Hoza, Jozef (referee) ; Slavík, Zdeněk (referee)
The main objective of our prospective experimental study was to develop a clinically relevant model of mechanical ventilation of healthy and damaged lungs ventilated for 12 hours. A widely recommended Vt=6ml/kg was used in comparison with commonly used Vt=10ml/kg. The study included a total of 52 piglets of domestic pig (Sus scrofa f. domestica) age 5-9 weeks. Piglets were divided into 3 groups: group B - Vt = 6ml/kg (11 piglets, 8 died), group C - Vt = 10ml/kg (21 piglets, 5 died) and group D - Vt= 50 ml + instillation of 50 ml saline into the trachea (6 piglets, 1 died). In the presented study we focused on the influence of mechanical ventilation on several organ systems as well as induction of systemic inflammatory reaction. By using higher tidal volume, we aimed to induce possible biotraumatic changes. The ventilator settings were deliberately not changed during the study, except the change of Fi02 values based on the Sp02 value. The first goal was to assess the influence of two different strategies of mechanical ventilation and induced lung injury on respiratory functions of the lungs and pulmonary mechanics. Piglets ventilated with Vt = 6ml/kg maintain the physiological oxemia, but the level of capnia increased over time with an adequate effect on acid-base balance. The highest mortality in...
|
|
| |
guest ::
