National Repository of Grey Literature 145 records found  beginprevious78 - 87nextend  jump to record: Search took 0.00 seconds. 
Biologically activ metabolites of plants VI . Alkaloids from Eschscholtzia Californica Cham. and their inhibiting activity to acetylcholinesterase.
Kittlerová, Zdeňka ; Cahlíková, Lucie (advisor) ; Siatka, Tomáš (referee)
M gr. Zdenka Kittlerová, roz. Horcicková Doc. Ing. L ucie Cahlíková, Ph.D. Biologically activ metabolites of plants VI. Alkaloids from Cham. and their inhibiting activity to acetylcholinesterase. Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Supervisor: Title of Rigorous Thesis: Keywords: , -methylcaryachine, argemonine, secondary plant metabolites, alkaloids, acetylcholinesterase, prolyloligopeptidase, Alzheimer's disease. Within the screening of plants that contains alkaloids inhibiting the activity of the human erythtocytic acetylcholinesterase and human serum butyrylcholinesterase Cham. ( ) was studied. This work connect to my diploma thesis (2011). The aim was to processing subfractions 36 and 39-45 obtained within the thesis. With chromatographic methods were isolated two alkaloids, which were based on MS and NMR studies identified as -methylcaryachine and argemonine. Both the isolated alkaloids were subjected to study for inhibitory activity against human erythrocyte AChE and BuChE serum. IC50 values were -methylcaryachine for AChE 498.00 ± 7.10 µM and BuChE was greater than 1000 µM. IC50 values were argemonine for AChE and BuChE to greater than 1000 µM. Obtained values were significantly higher than the IC50...
Natural compounds and their biological activity VII. Screening of selected alkaloidal plant species for cholinesterase aktivity.
Hovorková, Jana ; Opletal, Lubomír (advisor) ; Siatka, Tomáš (referee)
60 ABSTRACT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate Mgr. Jana Hovorková Consultant Prof. RNDr. Lubomír Opletal, CSc. Title of Thesis Natural compounds and their biological activity VII. Screening of selected alkaloidal plant species for cholinesterase aktivity. Within the systematic studies of plants producing metabolites that inhibit human erythrocytal AChE and serum BuChE, summary ethanolic extracts (S) and alkaloidal ethyl acetate extracts (B) of some plant species from the families Rutaceae (Dictamnus albus, root; Dictamnus dasycarpus, root bark; Evodia rutaecarpa, fruit; Phellodendron chinense, bark; Zanthoxylum bungeanum, pericarp; Zanhtoxylum nitidum, wood) and Nelumbonaceae (Nelumbo nucifera, embrya, seeds, leaves, stamen) were studied using Ellman's method. Among extracts of S-type, the highest inhibitory activity (μg/ml) was found in Evodia rutaecarpa (AChE 90.2 ± 11.1; BuChE 5.2 ± 0.6) and Phellodendron chinense (AChE 2.2 ± 0.2; BuChE 59.7 ± 6.4). In alkaloidal extracts of B-type, the enzymes were inhibited most by the compounds of Evodia rutaecarpa (AChE 9.8 ± 0.9; BuChE 9.7 ± 0.9) and of some morphological parts of Nelumbo nucifera: germs (AChE 45.9 ± 4.1; BuChE 11.6 ± 1.1) and leaves (AChE 59.2 ± 6.0; BuChE...
Iron-chelating acitivity of selected alkaloids III.
Dočekalová, Linda ; Macáková, Kateřina (advisor) ; Siatka, Tomáš (referee)
Dočekalová L., Iron-chelating activity of selected alkaloids III, Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical botanic and ecology, Hradec Králové, 2016, 60 pp. Iron is important trace element necessary for human organism. When excess it develops free radicals and structures of organism are harmed. It can lead to organ failure. Excess is solved with chelators, the most used is deferoxamine. But his terapeutic regimen is disadvantageous and it has a lot of side effects. That's reason for searching for substances with better features. This paper focuses on studium of iron-chelating activity of selected alkaloids from family Amaryllidaceae. Concretely, it's galanthamine, undulatine, buphanisine, caranine, 1-O- acetylbulbisine, homolycorine, tazettine, galanthamine, chlidanthine, ambelline, haemanthamine, haemanthidine, hamayne, 9-O-demethylgalanthine and lycoramine. As standard for comparison of activity was used deferoxamine. Activity was measured by spectrophotometer. As indicator was used ferrozine. Chelatation was measured for ferrous ionts and for total iron, where hydroxylamine as reduction agent was used. Ferrous ionts was measured also with pH alteration. Highest activity reached deferoxamine in all executed measurements. In...
Plant tissue culture of Juniperus virginiana L. as perspective source of podophyllotoxin
Srbová, Lenka ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Student: Lenka Srbová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: Tissue culture of Juniperus virginiana L. as a promising source of podophyllotoxin. The diploma thesis deals with the cultivation of Juniperus virginiana tissue cultures. A growth was observed at Juniperus virginiana two years old culture (variety 'Glauca' and 'Grey Owl') after adding various concentrations of phenylalanine (1 mmol.l-1, 10 mmol.l-1 and 100 mmol.l-1 ) at selected time intervals. The results show that the highest increase in callus fresh weight was detected at Juniperus virginiana variety 'Grey Owl', particularly on the 14th day after adding 10 mmol.l-1 phenylalanine. Suspension culture was successfully derived from the Juniperus virginiana two years old callus culture (variety 'Glauca').
Cationic antimicrobial peptides as potential drugs
Zach, Jaroslav ; Opletalová, Veronika (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Zach Jaroslav Supervisor: Doc. RNDr. Veronika Opletalová, Ph. D. Title of Diploma Thesis: Cationic antimicrobial peptides as potential drugs With the rapid rise in the emergence of bacterial strains resistant to multiple classes of antimicrobial agents, there is an urgent to need to develop novel antimicrobial therapies to combat these pathogens. Antimicrobial peptides (AMPs) are small, predominantly cationic ribosomally synthesized peptides, which permealize biological membranes. The assets of these peptides in clinical application include their potential for broad-spectrum activity, rapid bactericidal activity and low propensity for resistance development. This article is focused on history, mechanism of action and types of peptides that are produced by various organisms. Given the extensive topic only cationic antimicrobial peptides are discussed.
Biological activity of plants metabolites. XXVII. Alkaloids of Fumaria officinalis L. and their effect on acetylcholinesterase and butyrylcholinesterase.
Hulcová, Daniela ; Opletal, Lubomír (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Daniela Hulcová Consultant: Prof. RNDr. Lubomír Opletal, CSc. Title of Diploma Thesis: Biological aktivity of plants metabolites. XXVII. Alkaloids of Fumaria officinalis L. and their effect on acetylcholinesterase and butyrylcholinesterase The summary ethanolic and diethylether extract were prepared from the herbs of a plant Fumaria officinalis L. We have obtained 201 fractions from this extract by column chromatography on the neutral Al2O3 (aluminium oxide). Joined fraction 68-76 were processed by thin layer chromatography, and 3 substances were obtained in pure state: DH-1, DH-2, DH-3. These 3 compounds were identified as protopine, (+)-fumariline and N- methylcorydaldine by the comparison with the literature and results of MS and NMR. These alkaloids were tested for the inhibitory activity against human erythrocytic acetylcholinesterase and plasmatic butyrylcholinesterase by Ellman`s method. The isolated alkaloids did not show any significant inhibitory activity (IC50, µM) compared with the standard galanthamine (IC50, µM; AChE 1,710 ± 0,065, BuChE 42,30 ± 1,30): protopin: AChE: 345,42 ± 31,12, BuChE: 239,66 ± 20,89, (+)-fumarilin: AChE: 2939,2 ± 309,41, BuChE: 330,62 ±...
Natural compounds and their biological activity X. Screening of selected alkaloids on inhibition of prolyloligopeptidase
Ibrmajerová, Lucie ; Opletal, Lubomír (advisor) ; Siatka, Tomáš (referee)
IX. ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Lucie Ibrmajerová Consultant: prof. RNDr. Lubomír Opletal, CSc. Title of thesis: Natural compounds and their biological activity X. Screening of selected alkaloids on inhibition of prolyloligopeptidase Alzheimer's disease is the most serious and incurable neurodegenerative disease, which incidence is increasing. For these reasons, the search for new therapeutic practices and especially medicines that would expand the portfolio of useful therapeutic agents. At the present time (based on EBM) two main groups of drugs come into consideration: cognitives (AChE, or BuChE inhibitors) and NMDA receptor antagonists. Although the causes of the disease are not known, partial pathophysiological processes that are applied in the development of the disease are known. The search for new drug candidates in other areas is subject of an emerging research. One of these areas is the search for inhibitors prolyl endopeptidase which can favorably be applied in processes related to memory and learning. In this work, 41 alkaloidal substances (isoquinoline and quinolizidine alkaloids, alkaloids of plants from the family Amaryllidaceae), which were previously studied on the inhibition of...
Biological activity of plant metabolites XXIX. Alkaloids from selected cultivars of Narcissus triandrus L. and their influence on acetylcholinesterase and butyrylcholinesterase
Farkašovský, Marek ; Opletal, Lubomír (advisor) ; Siatka, Tomáš (referee)
Farkašovský M.: Biological activity of plant metabolites XXIX. Alkaloids of selected cultivars of Narcissus triandrus L. and their influence on acetylcholinesterase and butyrylcholinesterase. Diploma thesis. Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of pharmaceutical botany and ecology, Hradec Králové 2015, 70 pages Screening of seven bulb samples of Narcissus genus was performed. It included cultivars Narcissus triandrus cv. Hawera, Narcissus triandrus cv. Ice Wings, Narcissus triandrus cv. Stint, Narcissus triandrus cv. Tresamble, Narcissus cyclamineus cv. February Gold, Narcissus cyclamineus cv. Greenlet and Narcissus cyclamineus cv. Itzim. Primary extract was prepared by boiling crushed bulbs in ethanol 95% and then it was condensated. After extraction in diethyl ether and ethyl acetate was pure alkaloid extract dried with air flow in water bath. Isolated alkaloid extracts were tested for their inhibition activity on acetylcholinesterase and butyrylcholinesterase. Also GC-MS analysis was provided to identify alkaloids. These alkaloids were identified with GC-MS analysis - epinorgalanthamine, galanthamine, galanthine, haemanthamine, hippeastrine, homolycorine, cherylline, incartine, lycoramine, lycoramine-acetate, lycorine, narwedine, neruscine, sanguinine...
Explant culture of Juniperus virginiana
Předota, Václav ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
1 ABSTRACT Charles University in Prague Faculty of pharmacy in Hradec Králové Department of pharmacognosy Student: Václav Předota Supervisor: PharmDr. Marie Kašparová, PhD. Title of diploma thesis: Plant tissue culture of Juniperus virginiana The derivation of callus cultures from leaves of Juniperus virginiana (varieties Hetzii, Glauca and Grey Owl) and determination of their growth curves were studied in this work. The cultures were cultivated at the temperature of 25 řC and light period of 16 hours light/8 hours dark on the Schenk and Hildebrandt medium with the addition of 3.0 mg.l-1 α-NAA and 0.2 mg. l-1 kinetin. It is clear from the growth curves, that all three varieties reached their maximum in growth on 35th day of the cultivation. The best results were achieved by variety Glauca.
Alkaloids of the family Amaryllidaceae and their biological activity 1.
Mišáková, Kamila ; Cahlíková, Lucie (advisor) ; Siatka, Tomáš (referee)
Mišáková K: Alkaloids of the Amaryllidaceae family and their biological activity 1. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2014, 63 p. The aim of the diploma thesis was a preparation of alkaloidal extract from Nerine Bowdenii to izolation of 2 pure alkaloids by column and thin layer chromatography. Subsequently these two alkaloids were subjected to structural MS and NMR analysis and tested for biological activity against human cholinesterases (HuAChE and HuBuChE) and for antioxidant and cytotoxic activity. Isolated substances were identified as ambelline and undulatine alkaloids, which have been already described previously in this plant. In the test for determining cholinesterase inhibitory activity of ambelline and undulatine the following values were measured; for ambelline: IC50, HuAChE = 169 ± 7 μM a IC50, HuBuChE = 985 ± 25 μM, for undulatine: IC50, HuAChE = 23,52 ± 1,19 μM a IC50, HuBuChE > 1000 μM. Galanthamin (IC50, HuAChE = 1,71 ± 0,007 μM, IC50, HuBuChE = 42,30± 1,30 μM), huperzin A (IC50, HuAChE = 0,033 ± 0,001 μM, IC50, HuBuChE > 1000 μM) and eserin (IC50, HuAChE = 0,063 ± 0,001 μM, IC50, HuBuChE = 0,130 ± 0,004 μM) were used like a positive controls. In the test for...

National Repository of Grey Literature : 145 records found   beginprevious78 - 87nextend  jump to record:
Interested in being notified about new results for this query?
Subscribe to the RSS feed.