|
|
Explant culture of Trifolium pratense L.
Křepelová, Jana ; Siatka, Tomáš (referee) ; Kašparová, Marie (advisor)
Jana Křepelová Explantat culture of Trifolium pratense L. A principal precondition for successful elicitation used to increase the production of secondary metabolites is, among other, finding a suitable elicitor, its concentration and the optimal period of time of action on the plant culture in vitro, which was the aim of the present thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of ferrous sulphate (in four concentrations) on the production of flavonoids and isoflavonoids in the suspension culture Trifolium pratense L. (variety DO-9 and variety Sprint) cultiveted on a Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 6- benzylaminopurine, at the temperature of 25o C and 16 hour light/ 8 hour dark period. The maximal content of flavonoids found by a photometric determination according to Pharmacopoeia Bohemica 2005 was demonstrated in the suspension culture Trifolium pratense L. (variety DO-9) (0,500 %) after 48 hour action of the elicitor of the 10 μmol concentration and in the suspension culture Trifolium pratense L. (variety Sprint) (0,415 %) also after 48 hours action 10 μmol concentration. The maximal content of isoflavonoids found by a HPLC metod was demonstrated in the suspension culture Trifolium pratense L....
|
|
|
Production of secondary metabolites in plant tissue cultures
Beránková, Petra ; Kašparová, Marie (referee) ; Siatka, Tomáš (advisor)
Production of secondary metabolites in plant tissue cultures The influence of ammonium cerium(IV) nitrate (0.055, 0.55, 5.5, 55 and 275 mg/l of medium) as a potential elicitor of scopoletin production in cell suspension cultures of Angelica archangelica L. was investigated. The cultures were cultivated in a liquid Murashige and Skoog nutrient medium supplemented with 2 mg/l 2,4-dichlorophenoxyacetic acid and 0.4 mg/l benzylaminopurine in the light or dark. The content of scopoletin was determined by high performance liquid chromatography with fluorometric detection. The elicitor treatment improved production of scopoletin. In the dark-grown cultures, the highest amounts of scopoletin in the medium as well as in cells were reached with a concentration of 0.55 mg/l, in comparison with non-elicited culture. In the light-grown cultures, the content of scopoletin was increased only in the medium with an elicitor concentration of 0.055 mg/l.
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
guest ::
