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Analytical evaluation of salicylaldehyde isonicotinoyl hydrazone derivatives
Schöngut, Michal ; Štěrbová, Petra (advisor) ; Pilařová, Pavla (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate Mgr. Michal Schöngut Consultant doc. PharmDr. Petra Štěrbová, Ph.D. Title of Thesis Analytical evaluation of salicylaldehyde isonicotinoyl hydrazone derivatives Následuje překlad abstraktu práce do anglického jazyka High performance liquid chromatography (HPLC) is one of the most modern and widely used analytical separation methods. Its advantage is that it enables qualitative and quantitative determination of substances even in a complex biological matrix. Biocompatible iron chelators are used in therapy primarily for the treatment of iron overload. However, due to their antioxidant and antiproliferative effects, they could also find application in the treatment of other diseases (Parkinson's and Alzheimer's disease, cancer). The biocompatible iron chelator salicylaldehyde isonicotinoyl hydrazone (SIH) from the group of aroylhadrazones was very promising, with low toxicity and significant antioxidant and cytoprotective effect. However, its use is complicated by the short half-life in plasma. Therefore, chelators were derived from its structure that are expected to have higher stability. The tested substances were:...
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Analytical evaluation of novel drug candidates from the group of iron chelators using HPLC I.
Schöngut, Michal ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Nowadays, high performance liquid chromatography (HPLC) belongs among the most modern and most frequently used analytical separation techniques. Biocompatible iron chelators are mainly used for the treatment of iron overload. Nevertheless, in the light of new information about physiological and patophysiological role of iron in organism, their potential use was widened into other diseases. Iron chelators were proved to have antioxidative, cardioprotective and cytostatic effects. Salicylaldehyde isonicotinoyl hydrazone is a biocompatible iron chelator, which also shows these activities. However, its short half life in plasma makes its use difficult. Nowadays, novel derivates of SIH were prepared to improve its stability in plasma. 2'-hydroxypropiophenone isonicotinoyl hydrazone (HPP-INH) is one of newly prepared SIH derivates. The aim of this study was to develop optimal chromatographic conditions for HPLC analysis of HPP-INH and to develop sample pretreatment method using precipitation for its analysis in plasma. The analysis was carried out on a Merck 250x4 mm column I.D. LiChrospher® 100, RP-18e (5 μm) protected with Purospher® 100, RP-18e (5 μm) guard column. The following mobile phase gradient condition was developed for HPP-INH purity evaluation: phosphate buffer (0,01 mol/l NaH2PO4 · 2 H20 water...
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