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The genome size determination in sturgeons using 2-D a 3-D image cytometry.
SRP, Jiří
The genome size of evolutionary polyploid, neopolyploid and hybrid sturgeons is well known for its high variability. Aim of this study was 1) to specify the genome size of polyploid and neopolyploid sturgeons using an analysis of 2-D and 3-D images of specifically stained cells nuclei, 2) to evaluate the samples of populations for cytogenetic analysis needs and thereafter, 3) to compare both methods and record the data either for next research or for negative selection from the broodstock. This test has been done at the laboratory of molecular, cellular and quantitative genetics, Faculty of Fisheries and Protection of Waters USB in Vodňany using all sturgeons spawners and using samples obtained from some foreign fish farms which cooperated with the faculty. The samples included A. ruthenus, A. baerii, A. stellatus, A. gueldenstaedtii and Huso huso, intentionally bred hybrids of A. gueldenstaedtii (8n) x A. baerii (12n), A. baerii (8n) x A. ruthenus (4n), A. gueldenstaedtii (8n) x A. baerii (10n) a A. gueldenstaedtii (8n) x A. ruthenus (4n). As methods have been chosen image cytometry and confocal microscopy which use image digitalization and subsequently its computer analysis. The genome size was measured from the size of specifically stained nuclei of erythrocytes in specimens sampled. Result of this study was measuring the genome size in sturgeons under study using different methods, recording the obtained data, description of spatial conformation changes of cell nucleus with increasing ploidy level and deduction of impact to their physiology and comparing the methods between each other. The conclusion is necessity of another sturgeons genome size determination , choice of the best methods for more effective search and research of non-standard individuals and subsequently an examination of their physiological differences.
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Polyploidy in sturgeons
SRP, Jiří
The aim was to study the theoretical backgrounds of sturgeon fish genetics, focusing on aspects of polyploidy cytogenetics and to learn basics of reproduction and rearing of sturgeon fish in conditions of Central European aquaculture. The work describes an overview of methods for determining ploidy levels in fish, methods of artificial reproduction, methods of fish marking, sampling and evaluation of population samples for cytogenetic analysis. The experimental part dealt with samples of different sturgeon species and their hybrids, and the effects of spontaneous polyploidy and interspecific hybridization on biodiversity conservation of these endangered species were discussed.
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