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Study of red yeast metabolism on molecular level
Roubalová, Monika ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
This master thesis is focused on the molecular characterization of the eight red yeasts species. For molecular characterisation, the most variable rDNA regions ITS1, 5,8S ITS2 and the region encoding the large ribosomal subunit (26S) were amplified. This long region of the yeasts DNA was sequenced and compared by NCBI database for identification. The red yeasts identification was confirmed by data from DGGE method. Another aim of this thesis was to select the best yeasts producer of carotenoids and triacylglycerols. Rhodosporidium toruloides was found as the best producer and, thus, this strain was subjected to random mutagenesis by UV irradiation. The results of the production of metabolites by R. toruloides were compared with mutant strains, which were also adapted to the glycerol and waste whey substrates. The mutant strain G33 was found as the best producer of total carotenoids with a yield of 7.14 mg.g-1 of biomass. The highest production of ergosterol was demonstrated by the mutant strain Y34, the ergosterol yield was 47.72 mg.g-1 of biomass. The wild type of R. toruloides was able to produce the highest amount of both carotene (2.42 mg.g-1 of biomass) and TAG (76.32 mg.g-1 of biomass) on glucose medium.
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Study of red yeast metabolism on molecular level
Roubalová, Monika ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
This master thesis is focused on the molecular characterization of the eight red yeasts species. For molecular characterisation, the most variable rDNA regions ITS1, 5,8S ITS2 and the region encoding the large ribosomal subunit (26S) were amplified. This long region of the yeasts DNA was sequenced and compared by NCBI database for identification. The red yeasts identification was confirmed by data from DGGE method. Another aim of this thesis was to select the best yeasts producer of carotenoids and triacylglycerols. Rhodosporidium toruloides was found as the best producer and, thus, this strain was subjected to random mutagenesis by UV irradiation. The results of the production of metabolites by R. toruloides were compared with mutant strains, which were also adapted to the glycerol and waste whey substrates. The mutant strain G33 was found as the best producer of total carotenoids with a yield of 7.14 mg.g-1 of biomass. The highest production of ergosterol was demonstrated by the mutant strain Y34, the ergosterol yield was 47.72 mg.g-1 of biomass. The wild type of R. toruloides was able to produce the highest amount of both carotene (2.42 mg.g-1 of biomass) and TAG (76.32 mg.g-1 of biomass) on glucose medium.
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Analysis of the development of beekeeping in the Pilsen region and its prospects
Roubalová, Monika ; Prášilová, Marie (advisor)
The thesis defines basic concepts of beekeeping and analyses the development of beekeeping in the Pilsner region. Individual parts describe the history of beekeeping, diseases of bees, production of honey and wax. One part is devoted to the evalution of honey prices per one kilogram for the period from 2004 to 2014 in the Pilsner region and this part us supplemented by statistical data with charts. In the last part, there will be summarized and evaluated the knowledge of the development of beekeeping and ist prospects in the Pilsner region.
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Analysis of the development of beekeeping in the Pilsen region and its prospects
Roubalová, Monika ; Prášilová, Marie (advisor)
The thesis defines basic concepts of beekeeping and analyses the development of beekeeping in the Pilsner region. Individual parts describe the history of beekeeping, diseases of bees, production of honey and wax. One part is devoted to the evalution of honey prices per one kilogram for the period from 2004 to 2014 in the Pilsner region and this part us supplemented by statistical data with charts. In the last part, there will be summarized and evaluated the knowledge of the development of beekeeping and ist prospects in the Pilsner region.
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