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Designing and testing of new metal nanosubstrates for biomolecular sensors based on surface-enhanced Raman scattering (SERS) spectroscopy
Peksa, Vlastimil ; Procházka, Marek (advisor) ; Matějka, Pavel (referee) ; Richter, Ivan (referee)
Title: Designing and testing of new metal nanosubstrates for biomolecular sensors based on surface-enhanced Raman scattering (SERS) spectroscopy Author: Vlastimil Peksa Department: Institute of Physics of Charles University Supervisor: doc. RNDr. Marek Procházka, Ph.D., Institute of Physics of Charles University Abstract: This experimental methodical work was aimed at the optimization of selected gold and silver substrates and their use in construction of SERS-based biosensors, including following practical application. Several types of substrates, fabricated via a combination of bottom-up techniques on solid surfaces, were tested. The properties of these substrates were examined with probe molecules, namely methylene blue, porphyrins and tryptophan, on a confocal Raman microspectrometer. Obtained findings about the influence of analyte application, objective focusing and internal intensity standard were exploited for optimization of measurement procedures with regard to sensitivity, accuracy and reproducibility. A method for quantitative detection of food dye azorubine (E 122) in commercially available drinks was developed, based on these findings. Its results have shown its potential as a pre-scan method for field application and preliminary testing. Keywords: Metal nanosubstrates, biomolecules,...
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Studium elektrického pole v detektorech záření pomocí Pockelsova jevu
Hakl, Michael ; Franc, Jan (advisor) ; Richter, Ivan (referee)
Study of electric field in radiation detectors by Pockels effect (Master Thesis) by Michael Hakl Abstract Cadmium Telluride (CdTe) is a convenient candidate for room tem- perature detection of X-ray and gama radiation due to 1.5 eV band- gap energy and high atomic mass. Since CdTe has the highest linear electro-optical coefficient among II-VI compounds, the detector rep- resents a Pockels cell. Transmittance of the crystal is modulated by the internal electric field. Processing of infrared camera photographs results in an electric field profile between biasing electrodes. The elec- tric field in semi-insulting CdTe is influenced with deep level traps causing charge polarization under the electrodes. Occupation of traps is dependent on metal-semiconductor interface. Relation of charge accumulation and band bending for gold and indium contacts was studied. Repolarization/depolarization induced by additional illumi- nation with sub/above bandgap excitation laser was observed and ex- ploited for determination of the deep level energy. Results obtained by the Pockels-effect method were supported with luminescence measure- ments. Correlation between the occurrence of deep levels and surface point defects was discovered. Keywords: Pockels electro-optical effect, Cadmium Telluride ra- diation detector, Electric field, Schottky...
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Complex diffractive structures for surface plasmon resonance sensors
Vala, Milan ; Homola, Jiří (advisor) ; Richter, Ivan (referee) ; Procházka, Marek (referee)
Title: Complex diffractive structures for surface plasmon resonance sensors Author: Milan Vala Institute of Photonics and Electronics ASCR, v.v.i. Supervisor of the doctoral thesis: Prof. Jiří Homola, Ph.D., DSc. Institute of Photonics and Electronics, ASCR, v.v.i. Abstract: Nowadays, biosensors based on surface plasmon resonance (SPR) present one of the most advanced label-free optical biosensor technologies. These sensors are based on the monitoring of refractive index changes in the vicinity of a plasmonic metal and enable both rapid and sensitive detection of various biological or chemical agents as well as the analysis of biomolecular interactions in real time. This doctoral thesis presents the results of research of advanced diffractive structures having benefits for SPR biosensor technology. In addition to a theoretical analysis, this work consists of the fabrication and characterization of selected diffractive structures, and furthermore, the development of novel types of SPR biosensors based on these structures. In particular, the results presented in this thesis include the development of a novel method of fabrication of periodic plasmonic arrays based on multiple-beam interference lithography, the development of compact high-resolution diffraction grating-based SPR devices, and development of...
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Pokročilé simulace fotonických struktur metodou FDTD
Vozda, Vojtěch ; Veis, Martin (advisor) ; Richter, Ivan (referee)
Finite-Difference Time-Domain method (FDTD) is based on numerical solution of Maxwell's equations, nowadays widely used for simulating optical response of photonic structures. This paper provides brief introduction to the FDTD method and several important extensions which make the basic code much more versatile. In order to broaden analysis of photonic structures, transfer matrix method (TMM) is also involved. The code is firstly tested using simple model structures which optical response might be compared with different numerical or even analytical approaches. Debugged code is used to improve photonic crystals for enhanced sensitivity of biosensing devices based on refractive index changes of sensed medium. Last but not the least, properties (sensitivity and Q-factor of resonant peak) of holey waveguide are investigated in one-, two- and three-dimensional simulation. It is shown here, that even this simple structure may compete with complex photonic crystals in the field of biosensors. Powered by TCPDF (www.tcpdf.org)
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Surface plasmons in optical microstructures and their sensor applications
Adam, Pavel ; Homola, Jiří (advisor) ; Brynda, Eduard (referee) ; Richter, Ivan (referee)
Title: Surface plasmons in optical microstructures and their sensor applications Author: Pavel Adam Institute: Institute of Photonics and Electronics AS CR, v.v.i., Department of Optical Sensors Supervisor of the doctoral thesis: doc. Ing. Jiří Homola, CSc., DSc. Abstract: This work is focused on the study of surface plasmon resonance (SPR) sensor platforms based on wavelength division multiplexing (WDM) of multiple surface plasmons (SPs). These sensors are based on advanced diffraction gratings supporting either conventional or Bragg-scattered SPs, which are simultaneously excited at different wavelengths. These SPs are studied both analytically and numerically using rigorous coupled-wave analysis and an integral approach. WDM of two and three SPs is presented and followed by the method for the analysis of the resolution, noise and cross-sensitivity. This method is employed to analyze the ability of different SPR sensor platforms (supporting WDM of two SPs) to discriminate refractive index (RI) changes in a thin layer at the sensor surface from background RI changes. The WDM SPR sensors based on advanced diffraction gratings prepared by interferometric holography are developed and tested in a model biosensing experiment consisting of the layer-by-layer growth of protein multilayers. The linear WDM of two...
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Calculation of optical response of photonic structures by FDTD method
Vozda, Vojtěch ; Veis, Martin (advisor) ; Richter, Ivan (referee)
FDTD method is based on Maxwell's equations and this thesis describe how to make these differential equations computer readable for numerical solution known as the Yee algorithm. Time step dependence on spatial step is examined here in order to obtain stable solution. Discrete Fourier trasform is defined to obtain frequency dependent transmission and reflection coefficients. Programmed simulation is tested on analytically solvable structures even on slightly more complex systems whose optical response was computed by other type of simulation. Finally photonic crystals and their application as biosensors are discussed. Particular shape of photonic crystal is examined in details (frequency spectrum dependence upon spatial resolution, inaccuracy in geometry, different compounds in holes, geometry modification).
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Plasmonic biosensors based on extraordinary optical transmission
Dršata, Martin ; Richter, Ivan (referee) ; Petráček, Jiří (advisor)
Tato diplomová práce se zabývá rigorózními simulacemi plazmonických biosenzorů založených na jevu zvýšené optické transmise. První část je věnována popisu fyzikálních jevů a poznatků, které tvoří základ pro studium vlastností plazmonických senzorů, a popisu výpočetní metody konečných prvků v časové oblasti, která je využita v této práci. Vlastní výsledky jsou uvedeny v další části, která se zabývá výzkumem citlivosti, rozlišení a dalších charakteristik zvoleného typu plazmonického sensoru, tvořeného sítí kruhových nanoděr v tenké zlaté vrstvě na substrátě nitridu křemíku, v závislosti na řadě jeho geometrických parametrů. Tyto závislosti jsou sledovány ve třech různých případech, a to senzoru umístěného ve vakuu, ponořeného ve vodě a v případě kdy je na zlatém povrchu umístěna tenká dielektrická vrstva, která reprezentuje přítomnost biomolekul uchycených na povrchu senzoru.
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