National Repository of Grey Literature 82 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Ovarian carcinomas - the influence of the Rho kinase family on cell migration
Barešová, Veronika ; Urbánková Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Author: Veronika Barešová Title: Ovarian carcinomas - the influence of Rho family kinases on cell migration Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Ovarian carcinomas are the deadliest group of gynecological malignancies. This is due to a high rate of metastasis and acquired drug resistance. Metastasis is characterized by a phenotypic change of cells from epithelial to mesenchymal, resulting in the loss of cell-cell and cell-extracellular matrix contacts and subsequent cell movement. This process is called epithelial-mesenchymal transition and has been shown to also play a role in cell resistance to cisplatin. The movement of individual cells is mediated by the Rho GTPase family and can be divided into mesenchymal and amoeboid types. The amoeboid type is characterized by the Rho/ROCK signaling pathway, while mesenchymal movement is mediated by the Rac/Cdc42 pathways. In my thesis, we investigated the differences in expression of selected proteins in both signaling pathways in ovarian carcinoma cells. Initially, we focused on comparing the A2780 and A2780cis cell lines, which are resistant to cisplatin. We monitored markers of amoeboid movement, RhoA and phosphorylated myosin, and markers of mesenchymal movement, Wave-2,...
Evaluation of muscle strength and endurance of lactating women
Melicharová, Nikola ; Kovařík, Miroslav (advisor) ; Rathouská, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Nikola Melicharová Supervisor of master thesis: PharmDr. Miroslav Kovařík, Ph.D. Title of master thesis: Evaluation of muscle strength and endurance of lactating women This work aims to evaluate the change in the parameters of muscle strength and endurance of the upper and lower limbs in women during breastfeeding. Compare these measured values between the right and left limbs. Another goal is to test the connection between dynamometric parameters and the intake of micronutrients in food supplements. 10 lactating women aged 29-39 took part in the measurement. The women were examined four times. The first examination L1 took place on average 29 ± 5 days postpartum, the second L2 93 ± 29, the third L3 179 ± 15, and the last measurement was L4 274 ± 3 days postpartum. A digital pinch/grip analyzer and digital myometer were used to measure muscle strength and endurance of the upper and lower limbs. We used the BCM - Body Composition Monitor device to measure fat-free mass. Weight was measured for women using a body analysis scale from Tanita. When evaluating the measured results, we discovered a statistically significant difference between the values of the maximum strength of the left hand...
Ovarian carcinomas - the influence of the Rho kinase family on cell migration
Barešová, Veronika ; Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Author: Veronika Barešová Title: Ovarian carcinomas - the influence of Rho family kinases on cell migration Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Ovarian carcinomas are the deadliest group of gynecological malignancies. This is due to a high rate of metastasis and acquired drug resistance. Metastasis is characterized by a phenotypic change of cells from epithelial to mesenchymal, resulting in the loss of cell-cell and cell-extracellular matrix contacts and subsequent cell movement. This process is called epithelial-mesenchymal transition and has been shown to also play a role in cell resistance to cisplatin. The movement of individual cells is mediated by the Rho GTPase family and can be divided into mesenchymal and amoeboid types. The amoeboid type is characterized by the Rho/ROCK signaling pathway, while mesenchymal movement is mediated by the Rac/Cdc42 pathways. In my thesis, we investigated the differences in expression of selected proteins in both signaling pathways in ovarian carcinoma cells. Initially, we focused on comparing the A2780 and A2780cis cell lines, which are resistant to cisplatin. We monitored markers of amoeboid movement, RhoA and phosphorylated myosin, and markers of mesenchymal movement, Wave-2,...
Immunohistochemical evaluation of profibrotic precursors in precision cut liver slices
Richter, Adam ; Němečková, Ivana (advisor) ; Rathouská, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Adam Richter Supervisor: RNDr. Ivana Němečková, Ph.D. Title of Bachelor thesis: Immunohistochemical evaluation of profibrotic precursors in precision cut liver slices Organ hepatic fibrosis is the most typical disease progression in chronic inflammatory diseases and overall contributes significantly to worldwide mortality regardless of other causes. Activated hepatic stellate cells (HSC), which are found in the space of Disse in the liver, are most often responsible for liver fibrotic processes, and they can undergo differentiation into the myofibroblasts, which then significantly participate in the formation of fibrotic tissue. A number of marker genes and proteins specific to these cells are commonly used to observe the activation and development of fibrotic processes and their evaluation. This bachelor's thesis was devoted to the study of the detection of activation of profibrotic precursors in precise cut liver slices (PCLS). The determination of the expression of selected proteins in mouse ultrathin liver sections was used. The expression of desmin, glial fibrillary acidic protein (GFAP) and α-smooth muscle actin (αSMA) was evaluated. Sections for the study of fibrosis were...
TRC105 effects on development of fibrosis in liver
Masoumali, Kimia ; Němečková, Ivana (advisor) ; Rathouská, Jana (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences TRC105 effects on development of fibrosis in liver Diploma thesis Hradec Králové 2022 Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisor: Dr. Ivana Nemeckova, Ph.D. Consultant: M.Sc. Samira Eissazadeh Author: Kimia Masoumali Title of the thesis: TRC105 effects on the development of fibrosis in liver The objective of the thesis Hepatic stellate cells (HSCs) become contractile and proinflammatory myofibroblasts due to constant damage activating α-SMA (ɑ-smooth muscle actin) and ECM (extracellular matrix) components. α-SMA is an isoform of vascular smooth muscle actins that is predominantly produced in vascular smooth muscle cells, is a particular marker for smooth muscle differentiation and hepatocyte activation and contributes to fibrogenesis. GFAP (glial fibrillar acidic protein) is a monomeric intermediate filament (IF) III protein most typically expressed by astrocytes however, the expression of GFAP in vivo in inert stellate cells of the liver has been recognized. It's been proven that GFAP levels in the liver of bile duct ligated mice were heightened. CRIP2 (Cysteine- rich intestinal protein 2) is a member of the LIM proteins...
Effect of diet on the development of liver damage of non-alcoholic steatohepatitis in mice
Hemmerová, Amálie ; Rathouská, Jana (advisor) ; Němečková, Ivana (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Bioanalytical laboratory diagnostics in healthcare Diploma thesis Author: Bc. Amálie Hemmerová Supervisor: PharmDr. Jana Rathouská, Ph.D. Title: Effect of diet on the development of liver damage of non-alcoholic steatohepatitis in mice This thesis deals with the study of non-alcoholic steatohepatitis (NASH), which is considered to be a very serious chronic liver disease. Non-alcoholic steatohepatitis is an advanced form of NAFLD (non-alcoholic fatty liver disease) and is characterized by the presence of hepatic steatosis and inflammation. NASH can be followed by complications such as liver cirrhosis or hepatocellular carcinoma. For NASH research, the first thing to do is to choose the right animal model and diet. In this regard, the CDAA-HFD dietary model, which was also specified in this work, appears to be a very effective model for the study of NASH and hepatocellular carcinoma. The aim of the present study was to investigate the effect of that dietary regimen on the development of hepatic damage of the character of non-alcoholic steatohepatitis. For this purpose, selected parameters of liver fibrosis were monitored in mice in which the mentioned damage was induced by the dietary regimen. The...
SARS-CoV-2 vaccination
Nguyenová, Quynh Trang ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Background: The aim of this Bachelor thesis is to present to the reader the possibilities of vaccination against the SARS-CoV-2 virus, which causes the COVID-19 disease. Describe the structure of the virus, etiopathogenesis of the disease and summarize information on individual types of vaccines. Main findings: Thanks to the SARS-CoV-2 vaccines, the virus is quickly recognized after intrusion into the body and massive immune response is ensured, which prevents the more serious or severe course of COVID-19. Conclusions: High vaccination coverage with SARS-CoV-2 vaccines is the only way to stop the pandemic caused by this virus, protecting those in at-risk populations (seniors, paramedics) whom COVID-19 could be lethal. Key words: COVID-19 disease, SARS-CoV-2, COVID-19 vaccines
Aptamers application in research and diagnostics
Neumanová, Alice ; Svobodová, Zuzana (advisor) ; Rathouská, Jana (referee)
The bachelor thesis deals with affinity molecules that are able to specifically bind to the target molecule. The first chapter is devoted to antibodies, i.e., affinity molecules of protein nature. It discusses their structure, development, production, and use. The main focus of the thesis is on aptamers, or short stretches of single-stranded DNA or RNA, which are often referred to as synthetic alternatives to antibodies. They have similar functions to antibodies but differ in structure, development, and production. Because of their similarity to antibodies, aptamers are used in various detection technologies to diagnose diseases. Aptamers are still in the research phase, but the first commercially available aptamer diagnostic kits are slowly starting to appear on the market. The final chapter compares the pros and cons of the two biggest rivals in the field of affinity molecules - antibodies and aptamers. Key words: aptamers, antibodies, nanoantibodies, affimers, diagnostics, research
Current strategies of allergy therapy
Hrabalová, Kateřina ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Laboratory diagnostics in healthcare Author: Kateřina Hrabalová Supervisor: PharmDr. Jana Rathouská, Ph.D. Title: Current strategies of allergy therapy Background: Due to their increasing incidence and prevalence, allergies represent a problem of contemporary medicine. The aim of this work is to summarize basic information regarding allergies and to create a comprehensive overview of their treatment options. Main findings: There are several methods of treatment: identification and subsequent elimination of the allergen, pharmacotherapy, allergen immunotherapy and biological treatment with monoclonal antibodies. Patient education and regimen are also an essential part of treatment. The success of therapy lies in the most effective combination of treatment methods to ensure the quality life of the allergic patient with a minimum of problems. Conclusions: Information was sought on the immune system in relation to allergies, its physiological functions and pathological reactions involving the mechanism of the allergic reaction. An overview of the treatment options for allergic diseases was created with a focus on therapy in general. Keywords: allergy, treatment, pharmacotherapy,...
Carotuximab effects on endoglin expression and signaling in liver fibrosis
Mohammadi, SeyedehNiloufar ; Nachtigal, Petr (advisor) ; Rathouská, Jana (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Biological and Medical science Carotuximab effect on endoglin expression and signaling in liver fibrosis Diploma thesis Seyedehniloufar Mohammadi Hradec Králové 2022 Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Consultant: M.Sc. Samira Eissazadeh Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D Student: Seyedehniloufar Mohammadi Title of the thesis: Carotuximab effects on endoglin expression and signaling in liver fibrosis Objective of the thesis Endoglin is a 180 kDa transmembrane glycoprotein that acts as a coreceptor for binding to the Transforming growth factor β (TGFβ) superfamily and is found in two forms in the blood: membrane endoglin (Eng) and soluble endoglin (sEng) circulating in the blood. It has been demonstrated that Eng might play an important role in the process of liver fibrosis, inflammation, and endothelial dysfunction. According to different studies, Eng can have prefibrotic or antifibrotic activity. The precise impact of Eng expression and signalling changes, and sEng levels during liver disorders are still unknown. Carotuximab, TRC105 (chimeric IgG1), is a therapeutic monoclonal antibody that binds to...

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